Rodoman V E, Korotaev A L, Laberko L A, Zarma A A, Rodoman G V
Urol Nefrol (Mosk). 1989 Mar-Apr(2):13-5.
A method has been developed for speedy determination of urinary microflora sensitivity to antibacterial drugs, which differs from other existing techniques by its simplicity and rapid realization. It is based on the capacity of micro-organisms as an aggregate of particles with a refraction coefficient, different from that of the environment, to increase its optic density by multiplication in a liquid nutrient medium in conditions of unlimited nutrition and space. Thus, increased optic density of a urinary specimen with an added antibacterial agent during incubation in a thermostat would indicate an increased count of micro-organism in the medium, i.e. the lack of sensitivity to the antibiotic in question. Optic density being unchanged under such conditions is evidence of the death (lack of growth) of the micro-organism population and, hence, its sensitivity to the anti-bacterial drug being assessed. The ultimate results being obtainable within 2 to 6 hrs and little effort involved are the advantages of this method over conventional techniques of the determination of urinary flora sensitivity to antibacterial drugs that are in current clinical use. Besides, adequate antibacterial treatment can already be initiated within the first day of admission where this test is used.
已开发出一种快速测定尿液微生物对抗菌药物敏感性的方法，该方法与其他现有技术的不同之处在于其操作简单且能快速实现。它基于微生物作为具有不同于环境折射系数的颗粒聚集体，在营养和空间无限的条件下于液体营养培养基中繁殖时增加其光密度的能力。因此，在恒温箱中孵育期间添加抗菌剂的尿液标本光密度增加表明培养基中微生物数量增加，即对所讨论的抗生素不敏感。在这种条件下光密度不变证明微生物群体死亡（无生长），因此证明其对所评估的抗菌药物敏感。与目前临床使用的常规尿液菌群对抗菌药物敏感性测定技术相比，该方法的优点是最终结果可在2至6小时内获得且工作量小。此外，在使用该检测的情况下，入院第一天即可开始进行充分的抗菌治疗。
