一个42个碱基对的双链DNA微环的构建。

Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, FRG.

Nucleic Acids Res. 1989 Jul 11;17(13):5163-72. doi: 10.1093/nar/17.13.5163.

A procedure for the construction of double stranded DNA microcircles is described that overcomes the natural limits of established circularization procedures. Starting with two synthetic oligonucleotides which are able to form dumbbell shaped structures, two subsequent ligation reactions yield a microcircle of double stranded DNA of 42 base pairs. This is by far the smallest circle of double stranded DNA yet described. These microcircles can be constructed in quantities required for high resolution structural analyses such as X-ray crystallography and NMR spectroscopy.

本文描述了一种构建双链DNA微环的方法，该方法克服了现有环化程序的天然限制。从能够形成哑铃状结构的两条合成寡核苷酸开始，通过两个连续的连接反应产生一个42个碱基对的双链DNA微环。这是迄今为止所描述的最小的双链DNA环。这些微环可以按照高分辨率结构分析（如X射线晶体学和核磁共振光谱学）所需的量进行构建。