母源性纳米颗粒在胚胎大脑中的主动扩散。
Active diffusion of nanoparticles of maternal origin within the embryonic brain.
机构信息
Neuroscience Center, University of Helsinki, FI-00014 Helsinki, Finland.
Division of Pharmaceutical Chemistry & Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.
出版信息
Nanomedicine (Lond). 2016 Oct;11(19):2471-81. doi: 10.2217/nnm-2016-0207. Epub 2016 Sep 13.
AIM
To investigate porous silicon (PSi) nanoparticles (NPs) behavior in the embryonic brain.
MATERIALS & METHODS: Fluorescently labeled PSi NPs were injected into the embryonic brains intraventricularly and to the mother intravenously (iv.). Brain histology from different time points up to 3 days was analyzed and live brains imaged with two-photon microscopy.
RESULTS
PSi NPs were able to penetrate 80% of the embryonic cortical depth. Particle motility was confirmed in real-time in vivo. PSi NPs were able to penetrate the embryonic cortex after either iv. maternal or intraventricular injection. No developmental of macromorphological changes or increased cell apoptosis was observed.
CONCLUSION
PSi NPs penetrate deep in the brain tissues of embryos after intraventricular injection and after iv. injection to the mother.
目的
研究多孔硅（PSi）纳米颗粒（NPs）在胚胎大脑中的行为。
材料与方法
荧光标记的 PSi NPs 被脑室内注射到胚胎脑内，以及静脉内（iv.）注射到母体。分析了不同时间点的脑组织学，并用双光子显微镜对活脑进行成像。
结果
PSi NPs 能够穿透胚胎皮质 80%的深度。实时活体证实了颗粒的运动性。PSi NPs 能够穿透胚胎皮质，无论是通过 iv. 母体或脑室内注射。未观察到明显的胚胎发育性宏观形态变化或增加的细胞凋亡。
结论
PSi NPs 可穿透脑室内注射和静脉内注射到母体后的胚胎脑组织深部。
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