Shiratori K, Watanabe S, Takeuchi T
Department of Medicine and Gastroenterology, Tokyo Women's Medical College, Japan.
Pancreas. 1989;4(4):452-8. doi: 10.1097/00006676-198908000-00010.
We investigated the effects of oleic acid in the duodenum on pancreatic exocrine secretion and plasma secretin, and determined the role of cholinergic dependence on pancreatic secretion and secretin release in response to oleic acid in anesthetized rats. Oleic acid emulsion (pH 6.5) in three different doses of 0.06, 0.25, and 1 mmol/h was infused intraduodenally for 1 h with or without intravenous administration of atropine in a dose of 100 micrograms/kg/h. Intraduodenal administration of oleic acid resulted in significant increases in pancreatic juice volume and bicarbonate output, in a dose-related manner (p less than 0.001). Plasma secretin concentration caused dose-dependent elevation (p less than 0.001) by oleic acid, which correlated very well with bicarbonate output in response to oleic acid (p less than 0.001). Atropine inhibited pancreatic secretion including juice volume and bicarbonate output stimulated by oleic acid in each dose, statistically significantly (p less than 0.05-0.01), but did not affect plasma secretin concentration. Thus, we conclude that oleic acid in the duodenum stimulates pancreatic secretion and endogenous secretin release in rats, and that secretin release is not influenced by the cholinergic tone, although pancreatic secretory response is inhibited significantly.
我们研究了十二指肠内油酸对胰腺外分泌及血浆促胰液素的影响，并确定了在麻醉大鼠中，胆碱能依赖性在胰腺分泌及促胰液素释放对油酸反应中的作用。将三种不同剂量（0.06、0.25和1 mmol/h）的油酸乳剂（pH 6.5）经十二指肠内输注1小时，输注时伴或不伴静脉注射剂量为100微克/千克/小时的阿托品。十二指肠内给予油酸导致胰液分泌量和碳酸氢盐排出量显著增加，且呈剂量依赖性（p < 0.001）。油酸使血浆促胰液素浓度呈剂量依赖性升高（p < 0.001），这与对油酸反应时的碳酸氢盐排出量密切相关（p < 0.001）。阿托品抑制了各剂量油酸刺激的胰腺分泌，包括胰液分泌量和碳酸氢盐排出量，具有统计学显著性（p < 0.05 - 0.01），但不影响血浆促胰液素浓度。因此，我们得出结论：十二指肠内的油酸刺激大鼠胰腺分泌和内源性促胰液素释放，尽管胰腺分泌反应受到显著抑制，但促胰液素释放不受胆碱能张力的影响。
