Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstraße 41, 6020, Innsbruck, Austria.
Wien Klin Wochenschr. 2017 Jun;129(11-12):435-441. doi: 10.1007/s00508-016-1057-y. Epub 2016 Sep 13.
Rapid microbial diagnostics is important for septicemic patients. The current gold standard is blood culture with consecutive pathogen identification and antimicrobial susceptibility testing. However, these culture-based methods need at least 48 h.The aim of this study was to compare Verigene (Nanosphere, Northbrook, IL, USA), a rapid hybridization-based method, with conventional culture-based methods for detection of pathogens and resistance markers from positive blood cultures of septic patients.In 85 of 100 tested blood culture samples (85 %), pathogen identification as well as resistance profile were identical in Verigene and conventional culture. In 4 %, discordant results were observed. In 9 %, conventional culture revealed a pathogen ID or resistance phenotype not included in the Verigene panel. In 2 % no Verigene result was available.In conclusion, Verigene offers the availability of fast and reliable pathogen identification and resistance profile determination, which may result in an earlier start of adequate antimicrobial treatment.
快速微生物诊断对于败血症患者非常重要。目前的金标准是连续进行病原体鉴定和抗菌药物敏感性试验的血液培养。然而，这些基于培养的方法至少需要 48 小时。本研究的目的是比较 Verigene（Nanosphere，美国北布鲁克），一种基于快速杂交的方法，与传统的基于培养的方法，用于检测败血症患者阳性血培养中的病原体和耐药标记物。在 100 个测试的血培养样本中的 85 个（85%）中，Verigene 和传统培养的病原体鉴定和耐药谱完全一致。在 4%的情况下，观察到不一致的结果。在 9%的情况下，传统培养显示出 Verigene 面板中未包含的病原体 ID 或耐药表型。在 2%的情况下，无法获得 Verigene 结果。总之，Verigene 提供了快速可靠的病原体鉴定和耐药谱确定，这可能导致更早开始适当的抗菌治疗。
