Dodémont M, De Mendonça R, Nonhoff C, Roisin S, Denis O
Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium,
Eur J Clin Microbiol Infect Dis. 2015 Mar;34(3):473-7. doi: 10.1007/s10096-014-2250-4. Epub 2014 Sep 27.
The Verigene Gram-Positive Blood Culture (BC-GP) Assay (Nanosphere Inc., Northbrook, IL) is a microarray-based test designed to rapidly identify directly from positive blood cultures multiple bacterial species and their antimicrobial resistance markers. Nonduplicate blood cultures from 118 patients admitted to Erasme Hospital were prospectively enrolled. All but six organisms were members of the panel (95.6 %). For the identification of pathogens and detection of the mecA gene, the agreement with routine methods was 87.6 % and 97.7 %, respectively. The performance of the BC-GP assay was lower with polymicrobial than with monomicrobial blood cultures. Another concern of the BC-GP assay was the misidentification of Streptococcus mitis as S. pneumoniae (3/8). The BC-GP assay is a rapid and accurate tool for the simultaneous detection of multiple sepsis-causing bacteria and resistant genes from blood cultures, which could have an impact on patient management and healthcare cost.
Verigene革兰氏阳性血培养（BC-GP）检测法（美国Nanosphere公司，伊利诺伊州北布鲁克）是一种基于微阵列的检测方法，旨在直接从阳性血培养物中快速鉴定多种细菌及其抗菌耐药标志物。前瞻性纳入了伊拉斯谟医院收治的118例患者的非重复血培养样本。除6种微生物外，其余均在检测范围内（95.6%）。对于病原体鉴定和mecA基因检测，与常规方法的一致性分别为87.6%和97.7%。与单一微生物血培养相比，BC-GP检测法在混合微生物血培养中的表现较差。BC-GP检测法的另一个问题是将缓症链球菌误鉴定为肺炎链球菌（8例中有3例）。BC-GP检测法是一种快速、准确的工具，可同时从血培养物中检测多种引起败血症的细菌和耐药基因，这可能会对患者管理和医疗成本产生影响。
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