Santolaria Pilar, Soler Carles, Recreo Pilar, Carretero Teresa, Bono Araceli, Berné José M, Yániz Jesús L
TECNOGAM research group, Environmental Sciences Institute (IUCA), Department of Animal Production and Food Sciences, University of Zaragoza, Huesca, Spain.
Department of Functional Biology and Physical Anthropology, University of Valencia, 46100 Burjassot, Valencia, Spain.
Asian J Androl. 2016 Nov-Dec;18(6):831-834. doi: 10.4103/1008-682X.186874.
This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH [35.6% of all motile spermatozoa], with circular trajectories [32.0%], and rapid with high ALH [32.4%]), and three morphometric subpopulations (large-round [33.9% of all spermatozoa], elongated [32.0%], and small [34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.
本研究旨在分析正常精子男性射精不同部分中的精子运动学和形态学亚群。在禁欲3 - 5天后，通过手淫收集了8名正常精子男性的精液，并分为三个部分。使用计算机辅助精子分析（CASA - Mot）对精子活力进行分析，以及使用荧光技术通过计算机辅助精子形态分析（CASA - Morph）对精子形态进行分析。对获得的运动学和形态学数据进行聚类和判别程序，以识别精子亚群。聚类程序将精子分为三个运动学亚群（低侧摆幅度的慢速精子[占所有活动精子的35.6%]、呈圆形轨迹的精子[32.0%]、高侧摆幅度的快速精子[32.4%]），以及三个形态学亚群（大圆形精子[占所有精子的33.9%]、细长形精子[32.0%]、小精子[34.10%]）。射精各部分中运动学精子亚群的分布不同（P < 0.001），在第二部分和第三部分中，低侧摆幅度的慢速运动精子百分比更高，而在第一部分中运动学精子亚群的分布更均匀。形态学精子亚群的分布在射精各部分中也不同（P < 0.001），第一部分中细长形精子较多，第三部分中小精子较多。结论是，射精各部分之间精子运动学和形态学亚群的分布存在重要差异，可能具有功能意义。
