Yániz J L, Palacín I, Vicente-Fiel S, Sánchez-Nadal J A, Santolaria P
TECNOGAM Research Group, Environmental Sciences Institute (IUCA), University of Zaragoza, Huesca, Spain.
TECNOGAM Research Group, Environmental Sciences Institute (IUCA), University of Zaragoza, Huesca, Spain.
Anim Reprod Sci. 2015 May;156:128-34. doi: 10.1016/j.anireprosci.2015.03.012. Epub 2015 Mar 21.
The aim of the present study was to investigate whether differences in field fertility of rams are reflected in differences in sperm morphometric and kinematic population structures. The association between sperm morphometric and kinematic subpopulations was also investigated. Ejaculates from 8 adult rams, 4 with high and 4 with low field fertility, were collected weekly using an artificial vagina over 6 consecutive weeks. Analyses of sperm motility using computer-assisted sperm analysis (CASA) and sperm nuclear morphometry using computer-assisted sperm morphometry-fluorescence were performed. Clustering procedures using the kinematic and morphometric data from high and low field fertility rams resulted in the classification of spermatozoa in three kinematic and three morphometric sperm subpopulations. The distribution of subpopulations between rams of high and low field fertility was significantly different (P<0.05), with higher percentages of spermatozoa exhibiting fast and linear movements and those with large and long nuclei in the high fertility group. However, these subpopulations were not correlated. Logistic regression analyses were also performed to evaluate the relative utility of sperm subpopulations to classify rams in high and low field fertility. Total progressive sperm motility and the proportion of large and long spermatozoa were identified as the most consistent indicators of fertility. It was concluded that high and low fertility rams had clear differences in morphometric and kinematic sperm subpopulations, and that the most consistent indicators of fertility were the total progressive motility and the proportion of spermatozoa with large and long head present in the ejaculate.
本研究的目的是调查公羊田间繁殖力的差异是否反映在精子形态计量学和运动学群体结构的差异上。还研究了精子形态计量学亚群与运动学亚群之间的关联。连续6周每周使用人工阴道收集8只成年公羊的精液，其中4只具有高田间繁殖力，4只具有低田间繁殖力。使用计算机辅助精子分析（CASA）对精子活力进行分析，并使用计算机辅助精子形态计量学-荧光法对精子核形态进行分析。利用高、低田间繁殖力公羊的运动学和形态计量学数据进行聚类分析，将精子分为三个运动学亚群和三个形态计量学亚群。高、低田间繁殖力公羊的亚群分布存在显著差异（P<0.05），高繁殖力组中表现出快速直线运动的精子以及具有大而长细胞核的精子百分比更高。然而，这些亚群之间没有相关性。还进行了逻辑回归分析，以评估精子亚群在区分高、低田间繁殖力公羊方面的相对效用。总渐进性精子活力以及大而长的精子比例被确定为最一致的繁殖力指标。得出的结论是，高、低繁殖力公羊在精子形态计量学和运动学亚群方面存在明显差异，最一致的繁殖力指标是总渐进性活力以及射精中存在的大头精子比例。
