Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology, 44919, South Korea.
Sci Rep. 2016 Sep 15;6:33485. doi: 10.1038/srep33485.
One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrio-and-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264.7 mouse macrophage cultures with each predator, but paled in comparison to those obtained with E. coli. This was true even though the latter strain was added at an 11.1-fold lower concentration (p < 0.01). Likewise, E. coli led to a significant (54%) loss in the Raw 264.7 murine macrophage viability while the predatory strains had no impact. Tests with various epithelial cells, including NuLi-1 airway, Caco2, HT29 and T84 colorectal cells, gave similar results, with E. coli inducing IL-8 production. The viabilities of the NuLi-1 and Caco-2 cells were slightly reduced (8%) when exposed to the predators, while T84 viability remained steady. In no cases did the predatory bacteria induce actin rearrangement. These results clearly demonstrate the gentle natures of predatory bacteria and their impacts on human cells.
治疗多重耐药感染的抗生素替代品之一是噬菌体和类似物（BALOs），这是一种已知可以攻击人体病原体的捕食性细菌。因此，在这项研究中，研究人员研究了四种细胞系（三种人类细胞和一种小鼠细胞）在暴露于不同捕食性细菌（Bdellovibrio bacteriovorus HD100、Bacteriovorus BY1 和 Bacteriovorax stolpii EB1）时的反应。每种捕食性细菌都能诱导 Raw 264.7 小鼠巨噬细胞培养物中 TNF-α 的产生，但与大肠杆菌相比，其水平要低得多。即使后一种菌株的添加浓度低 11.1 倍（p < 0.01），也是如此。同样，大肠杆菌导致 Raw 264.7 小鼠巨噬细胞活力显著降低（54%），而捕食性菌株则没有影响。对各种上皮细胞（包括 NuLi-1 气道、Caco2、HT29 和 T84 结肠癌细胞）的测试也得到了类似的结果，大肠杆菌诱导了 IL-8 的产生。当暴露于捕食性细菌时，NuLi-1 和 Caco-2 细胞的活力略有降低（8%），而 T84 细胞的活力保持稳定。在任何情况下，捕食性细菌都不会诱导肌动蛋白重排。这些结果清楚地表明了捕食性细菌的温和特性及其对人类细胞的影响。
