Sussmann Rodrigo A C, de Moraes Marcilio M, Cebrián-Torrejón Gerardo, Porta Exequiel O, Doménech-Carbó Antonio, Yamaguchi Lydia F, Katzin Alejandro M, Kato Massuo J
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Oct 15;1033-1034:368-371. doi: 10.1016/j.jchromb.2016.09.011. Epub 2016 Sep 8.
Phylloquinone is a redox active naphthoquinone involved in electron transport in plants. The function of this reduced form remains unclear due to its instability, which has precluded detection. Herein, a simple method that permits the stabilization of the reduced form of phylloquinone by di-O-methylation and HPLC detection is described.
叶绿醌是一种参与植物电子传递的具有氧化还原活性的萘醌。由于其不稳定,这种还原形式的功能仍不清楚,这使得其检测受到阻碍。本文描述了一种通过二 - O - 甲基化稳定叶绿醌还原形式并进行高效液相色谱检测的简单方法。