Isshiki H, Suzuki Y, Yonekubo A, Hasegawa H, Yamamoto Y
Central Research Institute, Meiji Milk Products Co., Ltd., Tokyo, Japan.
J Dairy Sci. 1988 Mar;71(3):627-32. doi: 10.3168/jds.S0022-0302(88)79600-9.
A high performance liquid chromatographic method for measuring vitamin K in human milk and cow milk is described. The K vitamins were extracted with n-pentane from enzymatic hydrolysate of milk, purified by semipreparative HPLC, and then analyzed by reversed-phase HPLC equipped with a dual electrochemical detector. The amount of phylloquinone and menaquinone-4 in human milk was 2.1 +/- .9 and 1.3 +/- 1 microgram/L, respectively (n = 23). A small amount of menaquinone-6 was detected in both human and cow milk.
描述了一种用于测定人乳和牛乳中维生素K的高效液相色谱法。用正戊烷从牛奶的酶水解物中提取K族维生素，通过半制备高效液相色谱法进行纯化，然后用配备双电化学检测器的反相高效液相色谱法进行分析。人乳中叶绿醌和甲基萘醌-4的含量分别为2.1±0.9和1.3±1微克/升（n = 23）。在人乳和牛乳中均检测到少量的甲基萘醌-6。
