Langenberg J P, Tjaden U R
J Chromatogr. 1984 Apr 27;289:377-85. doi: 10.1016/s0021-9673(00)95103-3.
The use of the previously described electrofluorimetric reaction detection method for phylloquinone (vitamin K1) was extended to the determination of vitamins K1 epoxide in human plasma. The limit of detection for vitamin K1 epoxide is 60 pg, corresponding to plasma concentrations of about 120 pg/ml. The isolation method involves a liquid-liquid extraction of the lipids from the plasma, followed by a clean-up with silica. Synthesized 2',3'- dihydrovitamin K1 is used as an internal standard. The method was applied to the study of the presence of vitamin K1 epoxide and phylloquinone in plasma samples from healthy volunteers and from patients undergoing anticoagulant therapy.
将先前描述的用于叶绿醌（维生素K1）的电荧光反应检测方法扩展到人体血浆中维生素K1环氧化物的测定。维生素K1环氧化物的检测限为60皮克，相当于血浆浓度约为120皮克/毫升。分离方法包括从血浆中进行脂类的液-液萃取，随后用硅胶进行净化。合成的2',3'-二氢维生素K1用作内标。该方法应用于研究健康志愿者和接受抗凝治疗患者的血浆样本中维生素K1环氧化物和叶绿醌的存在情况。
