Department of Nanobiotechnology, Institute of Biotechnology, Brandenburgische Technische Universität Cottbus-Senftenberg , 01968 Senftenberg, Germany.
Anal Chem. 2016 Oct 18;88(20):10206-10214. doi: 10.1021/acs.analchem.6b02878. Epub 2016 Sep 26.
An ultrasensitive analytical method for direct detection of single nanoparticles in complex environment is described. The method relies on the wide-field surface plasmon microscopy (SPM). The suppression of matrix effects is achieved by image analysis based on the template matching. First, characteristic SPM images of nanoparticles are collected in aqueous suspensions. Then the detection of nanoparticles in complex environment is performed using template matching. Quantification and characterization of nanoparticles size was demonstrated at subppb level (∼100 pg/mL) in such complex media as wines, fruit juices, or cosmetic formulation. Visualization of the nanoparticles is performed in real time. The method does not require any sample pretreatment. If the minimally acceptable adsorption rate is defined as one nanoparticle to the whole sensor surface per few seconds, the working range of the method is ∼10 to 10 nanoparticles per mL.
本文描述了一种用于在复杂环境中直接检测单颗粒的超灵敏分析方法。该方法依赖于宽场表面等离子体显微镜（SPM）。通过基于模板匹配的图像分析来实现对基质效应的抑制。首先，在水悬浮液中收集纳米颗粒的特征 SPM 图像。然后，使用模板匹配进行复杂环境中的纳米颗粒检测。在葡萄酒、果汁或化妆品制剂等复杂介质中，以亚 ppb 级（约 100 pg/mL）的浓度实现了纳米颗粒大小的定量和表征。实时可视化纳米颗粒。该方法不需要任何样品预处理。如果将最小可接受的吸附速率定义为每几秒一个纳米颗粒到整个传感器表面，则该方法的工作范围约为每毫升 10 到 10 个纳米颗粒。
