Harrison G H, Balcer-Kubiczek E K
Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD 21201.
Ultrasound Med Biol. 1989;15(4):335-40. doi: 10.1016/0301-5629(89)90045-8.
C3H/10T1/2 cells in suspension were assayed using an initiation-promotion protocol for neoplastic transformation induced by continuous-wave ultrasound. Cells were insonated at 1.765 MHz for 40 min. Two ultrasonic intensities were used: 1.3 and 2.6 W/cm2 spatial average. The first intensity was found to be noncytotoxic; the second resulted in immediate lysis of 20% of the cells, followed by the clonogenic survival of 64% of the remaining cells. Ultrasound was delivered alone or in combination with X-rays (2 Gy, 240 kVp given before ultrasound), and/or 12-O-tetradecanoyl-phorbol-13-acetate (TPA, 0.1 microgram/ml post-irradiation). Under all treatment conditions, there was no effect of ultrasound on transformation at the 95% confidence level.
使用连续波超声诱导肿瘤转化的启动-促进方案对悬浮状态的C3H/10T1/2细胞进行检测。细胞在1.765兆赫兹频率下超声处理40分钟。使用了两种超声强度：空间平均强度分别为1.3和2.6瓦/平方厘米。发现第一种强度无细胞毒性；第二种强度导致20%的细胞立即裂解，其余64%的细胞克隆存活。超声单独使用或与X射线（2戈瑞，超声处理前给予240千伏峰值）和/或12-O-十四酰佛波醇-13-乙酸酯（TPA，照射后0.1微克/毫升）联合使用。在所有处理条件下，在95%置信水平下超声对转化无影响。
