Simon Coraline, Onghena Matthias, Covaci Adrian, Van Hoeck Els, Van Loco Joris, Vandermarken Tara, Van Langenhove Kersten, Demaegdt Heidi, Mertens Birgit, Vandermeiren Karin, Scippo Marie-Louise, Elskens Marc
Departement of Food Science, University of Liège - FARAH-Veterinary Public Health, Quartier Vallée 2, Avenue de Cureghem 10, Sart Tilman B43bis, 4000 Liège, Belgium.
Toxicological Centre, Faculty of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk-Antwerp, Belgium.
Toxicol In Vitro. 2016 Dec;37:121-133. doi: 10.1016/j.tiv.2016.09.008. Epub 2016 Sep 12.
Endocrine activity of 65 compounds migrating from polycarbonate replacement plastic baby bottles was assessed using in vitro cell based assays (reporter gene assays) involving 7 nuclear receptors, i.e. human steroid hormones receptors (oestrogen, androgen, progesterone and glucocorticoid receptors), human thyroid beta and peroxisome proliferator-activated gamma receptors, and the mouse aryl hydrocarbon receptor. The chemicals were tested at 4 concentrations ranging from 0.001mM to 1mM. Only twelve chemicals did not show any activity towards any of the nuclear receptors, while fifty three compounds showed a possible endocrine activity. Most of the agonistic activities were observed towards the oestrogen receptor while the PPARγ was the target for most of the recorded antagonistic activities. Agonistic activities were recorded for several phthalates, benzophenones, aromatic hydrocarbons and phenols, while compounds such as benzaldehydes, ketones and esters of fatty acid showed antagonistic activities. Thirty five chemicals were able of agonistic activities on 1 to 4 receptors and antagonistic activities were recorded for 35 compounds as well, towards 1 to 7 receptors. Sixteen compounds were able of both agonistic and antagonistic activities, but not on the same receptors, except in 2 cases for the oestrogen receptor and 4 cases for the PPARγ.
使用基于细胞的体外试验（报告基因试验），涉及7种核受体，即人类甾体激素受体（雌激素、雄激素、孕激素和糖皮质激素受体）、人类甲状腺β受体和过氧化物酶体增殖物激活γ受体，以及小鼠芳烃受体，评估了从聚碳酸酯替代塑料婴儿奶瓶中迁移出的65种化合物的内分泌活性。这些化学物质在0.001mM至1mM的4种浓度下进行了测试。只有12种化学物质对任何核受体均未表现出任何活性，而53种化合物显示出可能的内分泌活性。大多数激动活性是针对雌激素受体观察到的，而PPARγ是大多数记录到的拮抗活性的靶点。几种邻苯二甲酸盐、二苯甲酮、芳烃和酚类记录到了激动活性，而苯甲醛、酮类和脂肪酸酯等化合物显示出拮抗活性。35种化学物质对1至4种受体具有激动活性，35种化合物对1至7种受体也记录到了拮抗活性。16种化合物具有激动和拮抗活性，但不是针对相同的受体，雌激素受体有2例除外，PPARγ有4例除外。
