Zhao Heyan, Xue Jianhua, Liu Junhua, Liu Yifei, Cheng Yinan
Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, P.R. China.
Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
Oncol Rep. 2016 Nov;36(5):2814-2822. doi: 10.3892/or.2016.5081. Epub 2016 Sep 12.
The present study aimed to investigate the effect of metastasis suppressor 1 (MTSS1) on the proliferation, migration and invasion of human H1299 non-small cell lung cancer cells and its clinical significance in non‑small cell lung cancer. The target gene MTSS1-overexpressing lentivirus (LV-MTSS1) was transfected into H1299 cells and expression of MTSS1 was detected at the mRNA and protein levels. Cell Counting Kit-8, wound healing and Transwell assays revealed that the migration and invasion activities were significantly suppressed by MTSS1, but that it had no effect on cell proliferation. In addition, MTSS1 expression in tissue microarrays including samples from 223 cases of non-small cell lung cancer was tested by immunohistochemistry to explore the correlation between MTSS1 and clinicopathological characteristics and prognosis. MTSS1 suppressed H1299 cell migration and invasion, and its expression level can be used as a new independent factor for determining the prognosis of non-small cell lung cancer.
本研究旨在探讨转移抑制因子1（MTSS1）对人H1299非小细胞肺癌细胞增殖、迁移和侵袭的影响及其在非小细胞肺癌中的临床意义。将靶向基因MTSS1过表达慢病毒（LV-MTSS1）转染至H1299细胞，并在mRNA和蛋白质水平检测MTSS1的表达情况。细胞计数试剂盒-8、伤口愈合和Transwell实验表明，MTSS1可显著抑制细胞的迁移和侵袭活性，但对细胞增殖无影响。此外，采用免疫组织化学方法检测了包括223例非小细胞肺癌样本的组织芯片中MTSS1的表达，以探讨MTSS1与临床病理特征及预后的相关性。MTSS1抑制H1299细胞的迁移和侵袭，其表达水平可作为判断非小细胞肺癌预后的一个新的独立因素。
