Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 34129, Republic of Korea; Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere 33720, Finland.
Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 34129, Republic of Korea; Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Republic of Korea.
Bioresour Technol. 2016 Nov;220:661-665. doi: 10.1016/j.biortech.2016.09.025. Epub 2016 Sep 7.
Effective enhancement of neutral lipid (especially triacylglycerol, TAG) content in microalgae is an important issue for commercialization of microalgal biorefineries. Pressure is a key physical factor affecting the morphological, physiological, and biochemical behaviors of organisms. In this paper, we report a new stress-based method for induction of TAG accumulation in microalgae (specifically, Chlorella sp. KR-1 and Ch. sp. AG20150) by very-short-duration application of mild pressure. Pressure treatments of 10-15bar for 2h resulted in a considerable, ∼55% improvement of the 10-100g/Lcells' TAG contents compared with the untreated control. The post-pressure-treatment increase of cytoplasmic TAG granules was further confirmed by transmission electron microscopy (TEM). Notwithstanding the increased TAG content, the total lipid content was not changed by pressurization, implying that pressure stress possibly induces rapid remodeling/transformation of algal lipids rather than de novo biosynthesis of TAG.
有效提高微藻中的中性脂质（特别是三酰基甘油，TAG）含量是微藻生物炼制商业化的一个重要问题。压力是影响生物形态、生理和生化行为的关键物理因素。在本文中，我们报告了一种新的基于压力的方法，通过短暂施加温和的压力来诱导微藻（特别是绿球藻 KR-1 和 Ch. sp. AG20150）中 TAG 的积累。与未处理的对照组相比，压力处理 10-15 巴 2 小时可使 10-100g/L 细胞的 TAG 含量提高约 55%。细胞质 TAG 颗粒在压力处理后的增加进一步通过透射电子显微镜（TEM）得到证实。尽管 TAG 含量增加，但总脂质含量没有因加压而改变，这意味着压力胁迫可能会诱导藻类脂质的快速重塑/转化，而不是 TAG 的从头生物合成。
