Gwangju Bio/Energy R&D Center, Korea Institute of Energy Research, Gwangju 61003, Republic of Korea; Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea.
Gwangju Bio/Energy R&D Center, Korea Institute of Energy Research, Gwangju 61003, Republic of Korea.
Bioresour Technol. 2019 Nov;292:121950. doi: 10.1016/j.biortech.2019.121950. Epub 2019 Aug 2.
In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in Chlorella sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via de novo fatty acid biosynthesis and metabolic transformation in the Chlorella cells.
本工作提出了一种新的基于应激的方法，通过温和的电刺激快速诱导小球藻中三酰基甘油 (TAG) 以及总脂肪酸和多不饱和脂肪酸的积累。当向藻细胞施加 31 mA 的阴极电流 (电压：4 V) 4 小时时，电处理细胞的 TAG 含量急剧增加到未处理对照的 2.1 倍。电处理细胞中多不饱和亚油酸 (C18:2n6) 和亚麻酸 (C18:3n3) 的含量也分别比未处理细胞高 36%和 57%。循环伏安法和各种生化分析表明，TAG 和脂肪酸的形成是通过小球藻细胞中新的脂肪酸生物合成和代谢转化来电刺激的。
