Purushothaman Kathiresan, Lau Doreen, Saju Jolly M, Musthaq Sk Syed, Lunny Declan Patrick, Vij Shubha, Orbán László
Reproductive Genomics Group, Temasek Life Sciences Laboratory , Singapore , Singapore.
Institute of Medical Biology, Agency for Science, Research and Technology , Singapore.
PeerJ. 2016 Aug 24;4:e2377. doi: 10.7717/peerj.2377. eCollection 2016.
Asian seabass (Lates calcarifer) is a food fish of increasing aquaculture importance. In order to improve our understanding on the digestive system and feeding of this species, morphological and histological features of the gut were studied. Morphologically, the Asian seabass gut is defined by a short and muscular esophagus, well-developed stomach and comparatively short intestine. Mucous secreting goblet cells reactive to PAS (Periodic Acid Schiff) and AB (Alcian Blue) stain were present throughout the esophagus. The stomach was sac-like and could be distinguished into the cardiac, fundic and pyloric regions. Gastric glands and mucus cells were predominately present in the cardiac and fundic regions. Five finger-like pyloric caeca were present between the stomach and intestine. The intestine was a short, tubular structure with no morphological differences between the various regions. Histologically, the intestinal regions were similar, the main difference being in the number of goblet cells that increased from anterior to posterior intestine, with 114 ± 9, 153 ± 7 and 317 ± 21 goblet cells in the anterior, mid and posterior regions, respectively. The intestinal epithelium stained positively for PAS, but the staining was stronger for acidic glycoproteins. The rectum was similar to intestine, except for increased goblet cell numbers (anterior rectum: 529 ± 26; posterior rectum: 745 ± 29). Gut morpho-histology did not respond to salinity changes, however, there was a significant reduction of mucosal height, goblet cell numbers and muscularis thickness upon food deprivation.
尖吻鲈（尖吻鲈属）是一种在水产养殖中重要性日益增加的食用鱼。为了增进我们对该物种消化系统和摄食的了解，对其肠道的形态学和组织学特征进行了研究。在形态学上，尖吻鲈的肠道由短而肌肉发达的食道、发育良好的胃和相对较短的肠道所界定。对过碘酸希夫（PAS）和阿尔辛蓝（AB）染色有反应的黏液分泌杯状细胞遍布整个食道。胃呈囊状，可分为贲门部、胃底部和幽门部。胃腺和黏液细胞主要存在于贲门部和胃底部。在胃和肠道之间有五个指状幽门盲囊。肠道是一个短的管状结构，不同区域之间在形态上没有差异。在组织学上，肠道各区域相似，主要区别在于杯状细胞的数量从肠道前部到后部增加，前部、中部和后部区域的杯状细胞数量分别为114±9、153±7和317±21个。肠道上皮对PAS染色呈阳性，但对酸性糖蛋白的染色更强。直肠与肠道相似，只是杯状细胞数量增加（直肠前部：529±26；直肠后部：745±29）。肠道形态组织学对盐度变化没有反应，然而，饥饿时黏膜高度、杯状细胞数量和肌层厚度会显著减少。
