Manzoor Atif, UlAbdin Zain, Webb Bruce A, Arif Muhammad Jalal, Jamil Amer
Department of Entomology, University of Agriculture Faisalabad, Pakistan.
Department of Entomology, University of Agriculture Faisalabad, Pakistan.
Comp Biochem Physiol Part D Genomics Proteomics. 2016 Dec;20:101-110. doi: 10.1016/j.cbd.2016.08.002. Epub 2016 Aug 28.
Venom is a key-factor in the regulation of host physiology by parasitic Hymenoptera and a potentially rich source of novel bioactive substances for biotechnological applications. The limited study of venom from the ectoparasitoid Bracon hebetor, a tiny wasp that attacks larval pest insects of field and stored products and is thus a potential insect control agent, has not described the full complement and composition of these biomolecules. To have a comprehensive picture of genes expressed in the venom glands of B. hebetor, a venom gland transcriptome was assembled by using next generation sequencing technologies followed by de novo assemblies of the 10.81 M sequence reads yielded 22,425 contigs, of which 10,581 had significant BLASTx hits to know genes. The majority of hits were to Diachasma alloeum, an ectoparasitoid from same taxonomic family, as well as other wasps. Gene ontology grouped the sequences into molecular functions in which catalytic activity with 42.2% was maximum, cellular components in which cells with 33.8% and biological processes among which metabolic process with 30% had the most representatives. In this study, we highlight the most abundant sequences, and those that are likely to be functional components of the venom for parasitization. Full length ORFs of Calreticulin, Venom Acid Phosphatase Acph-1 like protein and arginine kinase proteins were isolated and their tissue specific expression was studied by RT-PCR. Our report is the first to characterize components of the B. hebetor venom glands that may be useful for developing control tools for insect pests and other applications.
毒液是寄生膜翅目昆虫调节宿主生理机能的关键因素，也是生物技术应用中潜在的新型生物活性物质的丰富来源。微小黄蜂麦蛾茧蜂是一种外寄生蜂，可攻击田间和储存产品中的害虫幼虫，因此是一种潜在的害虫防治剂，但对其毒液的研究有限，尚未描述这些生物分子的完整组成。为全面了解麦蛾茧蜂毒腺中表达的基因，利用下一代测序技术组装了毒腺转录组，对1081万个序列读数进行从头组装，得到22425个重叠群，其中10581个与已知基因有显著的BLASTx比对结果。大多数比对结果是与同分类科的外寄生蜂异痣金小蜂以及其他黄蜂的基因。基因本体论将这些序列分为分子功能（其中催化活性占比最大，为42.2%）、细胞成分（其中细胞占33.8%）和生物学过程（其中代谢过程占30%，代表序列最多）。在本研究中，我们重点介绍了最丰富的序列，以及那些可能是寄生毒液功能成分的序列。分离了钙网蛋白、毒液酸性磷酸酶Acph - 1样蛋白和精氨酸激酶蛋白的全长开放阅读框，并通过RT - PCR研究了它们的组织特异性表达。我们的报告首次对麦蛾茧蜂毒腺的成分进行了表征，这些成分可能有助于开发害虫防治工具及其他应用。
