Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Spinoff XFlies s.r.l., University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Biomolecules. 2023 Oct 19;13(10):1547. doi: 10.3390/biom13101547.
The larval stages of the tobacco budworm, (Fabricius) (Lepidoptera: Noctuidae), are parasitized by the endophagous parasitoid wasp, (Viereck) (Hymenoptera: Braconidae). During the injections of eggs, this parasitoid wasp also injects into the host body the secretion of the venom gland and the calyx fluid, which contains a polydnavirus ( BracoVirus: BV) and the Ovarian calyx fluid Proteins (OPs). The effects of the OPs on the host immune system have recently been described. In particular, it has been demonstrated that the OPs cause hemocytes to undergo a number of changes, such as cellular oxidative stress, actin cytoskeleton modifications, vacuolization, and the inhibition of hemocyte encapsulation capacity, which results in both a loss of hemocyte functionality and cell death. In this study, by using a combined transcriptomic and proteomic analysis, the main components of ovarian calyx fluid proteins were identified and their possible role in the parasitic syndrome was discussed. This study provides useful information to support the analysis of the function of ovarian calyx fluid proteins, to better understand parasitization success and for a more thorough understanding of the components of ovarian calyx fluid proteins and their potential function in combination with other parasitoid factors.
烟草夜蛾（Fabricius）（鳞翅目：夜蛾科）的幼虫阶段被内寄生性寄生蜂，（Viereck）（膜翅目：Braconidae）寄生。在产卵时，这种寄生蜂还会将毒液腺的分泌物和花萼液注入宿主体内，其中包含一种多DNA 病毒（BracoVirus：BV）和卵巢花萼液蛋白（OPs）。OPs 对宿主免疫系统的影响最近已被描述。特别是，已经证明 OPs 会导致血细胞发生多种变化，如细胞氧化应激、肌动蛋白细胞骨架修饰、空泡化和血细胞包封能力的抑制，从而导致血细胞功能丧失和细胞死亡。在这项研究中，通过结合转录组学和蛋白质组学分析，鉴定了卵巢花萼液蛋白的主要成分，并讨论了它们在寄生综合征中的可能作用。本研究为分析卵巢花萼液蛋白的功能提供了有用的信息，有助于更好地理解寄生成功，并更全面地了解卵巢花萼液蛋白及其与其他寄生因子结合的潜在功能。
