TUC338过表达导致舌鳞状细胞癌细胞在体外增殖增强和凋亡减少。
TUC338 Overexpression Leads to Enhanced Proliferation and Reduced Apoptosis in Tongue Squamous Cell Carcinoma Cells In Vitro.
作者信息
Ouyang Ke-Xiong, Zou Rui, Liang Jun, Bai Zhi-Bao, Li Zhi-Qiang, Zhao Jian-Jiang
机构信息
Professor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Guangzhou Medical University, Guangzhou, China.
Resident Doctor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Guangzhou Medical University, Guangzhou, China.
出版信息
J Oral Maxillofac Surg. 2017 Feb;75(2):423-428. doi: 10.1016/j.joms.2016.08.009. Epub 2016 Aug 24.
PURPOSE
Long noncoding RNAs are closely related to the development of tumors. In this study, we explored the contribution of the long noncoding RNA TUC338 to cellular processes in tongue squamous cell carcinoma (TSCC).
MATERIALS AND METHODS
First, we detected TUC338 expression using quantitative reverse transcription-polymerase chain reaction in 25 patients. Then, we transfected a short hairpin RNA to silence TUC338 expression in the CAL-27 and SCC-9 cell lines. Tumor cell growth was determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, and apoptosis and cell-cycle analyses were performed via flow cytometry.
RESULTS
The results indicated that TUC338 was overexpressed in TSCCs (P < .05). In addition, silencing TUC338 in CAL-27 and SCC-9 cells inhibited cell growth and increased apoptosis significantly in vivo (P < .05).
CONCLUSIONS
Long noncoding RNA TUC338 overexpression leads to enhanced proliferation and reduced apoptosis in TSCC.
目的
长链非编码RNA与肿瘤的发生发展密切相关。在本研究中，我们探讨了长链非编码RNA TUC338在舌鳞状细胞癌（TSCC）细胞进程中的作用。
材料与方法
首先，我们采用定量逆转录-聚合酶链反应检测了25例患者中TUC338的表达。然后，我们转染短发夹RNA以沉默CAL-27和SCC-9细胞系中TUC338的表达。通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四氮唑溴盐（MTT）法测定肿瘤细胞生长情况，并通过流式细胞术进行凋亡和细胞周期分析。
结果
结果表明，TUC338在TSCC中高表达（P <.05）。此外，在CAL-27和SCC-9细胞中沉默TUC338可显著抑制体内细胞生长并增加凋亡（P <.05）。
结论
长链非编码RNA TUC338的过表达导致TSCC增殖增强和凋亡减少。
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