State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Science Research Center, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Protein Cell. 2016 Nov;7(11):792-803. doi: 10.1007/s13238-016-0310-5. Epub 2016 Sep 8.
MRG proteins are conserved during evolution in fungi, flies, mammals and plants, and they can exhibit diversified functions. The animal MRGs were found to form various complexes to activate gene expression. Plant MRG1/2 and MRG702 were reported to be involved in the regulation of flowering time via binding to H3K36me3-marked flowering genes. Herein, we determined the crystal structure of MRG701 chromodomain (MRG701). MRG701 forms a novel dimerization fold both in crystal and in solution. Moreover, we found that the dimerization of MRG chromodomains is conserved in green plants. Our findings may provide new insights into the mechanism of MRGs in regulation of gene expression in green plants.
MRG 蛋白在真菌、苍蝇、哺乳动物和植物的进化过程中是保守的，它们可以表现出多样化的功能。动物 MRG 被发现形成各种复合物来激活基因表达。据报道，植物 MRG1/2 和 MRG702 通过与 H3K36me3 标记的开花基因结合参与开花时间的调节。在此，我们确定了 MRG701 色氨酸结构域（MRG701）的晶体结构。MRG701 在晶体和溶液中均形成一种新颖的二聚化折叠。此外，我们发现 MRG 色氨酸结构域的二聚化在绿色植物中是保守的。我们的发现可能为 MRG 在调控绿色植物基因表达中的机制提供新的见解。
