Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Collaboration Innovation Center for Cell Signaling, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Nat Commun. 2019 Mar 21;10(1):1303. doi: 10.1038/s41467-019-09310-x.
In plants, flowering time is controlled by environmental signals such as day-length and temperature, which regulate the floral pathway integrators, including FLOWERING LOCUS T (FT), by genetic and epigenetic mechanisms. Here, we identify an H3K27me3 demethylase, JUMONJI 13 (JMJ13), which regulates flowering time in Arabidopsis. Structural characterization of the JMJ13 catalytic domain in complex with its substrate peptide reveals that H3K27me3 is specifically recognized through hydrogen bonding and hydrophobic interactions. Under short-day conditions, the jmj13 mutant flowers early and has increased FT expression at high temperatures, but not at low temperatures. In contrast, jmj13 flowers early in long-day conditions regardless of temperature. Long-day condition and higher temperature induce the expression of JMJ13 and increase accumulation of JMJ13. Together, our data suggest that the H3K27me3 demethylase JMJ13 acts as a temperature- and photoperiod-dependent flowering repressor.
在植物中，开花时间受环境信号（如日长和温度）的控制，这些信号通过遗传和表观遗传机制调节花途径整合因子，包括 FLOWERING LOCUS T (FT)。在这里，我们鉴定出一种 H3K27me3 去甲基化酶，JUMONJI 13 (JMJ13)，它调节拟南芥的开花时间。JMJ13 催化结构域与其底物肽复合物的结构表征表明，H3K27me3 通过氢键和疏水相互作用特异性识别。在短日照条件下，jmj13 突变体开花早，在高温下 FT 表达增加，但在低温下不增加。相比之下，jmj13 在长日照条件下无论温度如何都会提前开花。长日照条件和较高的温度诱导 JMJ13 的表达，并增加 JMJ13 的积累。总之，我们的数据表明，H3K27me3 去甲基化酶 JMJ13 作为一种温度和光周期依赖性的开花抑制因子发挥作用。
