Yoo Yeon-Jee, Oh Joung-Hwan, Lee WooCheol, Woo Kyung Mi
Department of Conservative Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Department of BK21 Plus Program, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
J Endod. 2016 Nov;42(11):1626-1632. doi: 10.1016/j.joen.2016.08.004. Epub 2016 Sep 14.
The purpose of this study was to ascertain the regenerative characteristics of apical papilla-derived cells (APDCs) from immature teeth with pulpal and periapical pathosis and thus to provide proof-of-principle evidence for further regenerative endodontic research.
Pulpal and periapical pathosis was induced in immature permanent double-rooted premolars of beagles, which were randomly assigned to experimental treatment groups: group AO (n = 14), pulp disruption and access left open; group PS (n = 14), supragingival plaque suspension-soaked cotton pellet was introduced, and access was sealed; and control (n = 7), untreated. The teeth were extracted at 2- and 4-week periods after experimental treatments. APDCs were cultured from the extracted teeth, and their cellular proliferation, differentiation characteristics, and stemness were assessed. The data were statistically analyzed.
After 4 weeks of intentional pulpal and periapical pathosis induction period, all teeth in group PS showed features of apical periodontitis with necrotic pulp, and their APDCs showed significantly increased proliferation rate and osteogenic/odontogenic differentiation capabilities (P < .05). The stemness was maintained in all APDCs, although the stem cell population was smaller in group PS at 2-week period when the inflammatory responses were most fulminant (P < .05).
The APDCs from immature teeth retained the regenerative characteristics with the differences according to their pulpal and periapical pathosis. The results of this study partly provide the evidence for regenerative endodontic research.
本研究的目的是确定患有牙髓和根尖周病变的未成熟牙齿根尖乳头来源细胞（APDCs）的再生特性，从而为进一步的牙髓再生研究提供原理性证据。
在比格犬未成熟的恒双根前磨牙中诱导牙髓和根尖周病变，将其随机分配到实验治疗组：AO组（n = 14），牙髓破坏且开髓后敞开；PS组（n = 14），引入龈上菌斑悬液浸泡的棉球，然后封闭开髓处；对照组（n = 7），不进行处理。在实验处理后2周和4周时拔牙。从拔除的牙齿中培养APDCs，并评估其细胞增殖、分化特性和干性。对数据进行统计学分析。
在有意诱导牙髓和根尖周病变4周后，PS组所有牙齿均表现出牙髓坏死的根尖周炎特征，其APDCs的增殖率和骨生成/牙生成分化能力显著增加（P <.05）。所有APDCs均保持干性，尽管在炎症反应最强烈的2周时，PS组的干细胞群体较小（P <.05）。
未成熟牙齿的APDCs保留了再生特性，且根据牙髓和根尖周病变情况存在差异。本研究结果部分为牙髓再生研究提供了证据。
