College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
Appl Microbiol Biotechnol. 2016 Oct;100(20):8693-9. doi: 10.1007/s00253-016-7829-5. Epub 2016 Sep 17.
Escherichia coli strain Nissle 1917 (EcN) has been used as a probiotic. Genetic engineering has enhanced the utility of EcN in several vaccine and pharmaceutical preparations. We discuss in this mini review the genetics and physical properties of EcN. We also discuss the numerous genetic engineering strategies employed for EcN-based vaccine development, including recombinant plasmid transfer, genetic engineering of cryptic plasmids or the EcN chromosome, EcN bacterial ghosts and its outer membrane vesicles. We also provide a current update on the progress and the challenges regarding the use of EcN in vaccine development.
大肠杆菌菌株 Nissle 1917（EcN）已被用作益生菌。基因工程增强了 EcN 在几种疫苗和药物制剂中的应用。在这篇综述中，我们讨论了 EcN 的遗传学和物理特性。我们还讨论了用于 EcN 疫苗开发的多种基因工程策略，包括重组质粒转移、隐性质粒或 EcN 染色体的基因工程、EcN 细菌噬菌体及其外膜囊泡。我们还提供了关于 EcN 在疫苗开发中应用的最新进展和挑战的信息。
