Pontigo Juan Pablo, Agüero María José, Sánchez Patricio, Oyarzún Ricardo, Vargas-Lagos Carolina, Mancilla Jorge, Kossmann Hans, Morera Francisco J, Yáñez Alejandro J, Vargas-Chacoff Luis
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Programa Doctorado en Ciencias, Mención Biología Celular y Molecular, Escuela de Graduados, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile.
Fish Shellfish Immunol. 2016 Nov;58:259-265. doi: 10.1016/j.fsi.2016.09.031. Epub 2016 Sep 15.
The NOD-like receptors (NLRs) were recently identified as an intracellular pathogen recognition receptor family in vertebrates. While the immune system participation of NLRs has been characterized and analyzed in various mammalian models, few studies have considered NLRs in teleost species. Therefore, this study analyzed the Atlantic salmon (Salmo salar) NLRC5. Structurally, Atlantic salmon NLRC5 presented leucine-rich repeat subfamily genes. Phylogenetically, NLRC5 was moderately conserved between S. salar and other species. Real-time quantitative PCR revealed NLRC5 expression in almost all analyzed organs, with greatest expressions in the head kidney, spleen, and hindgut. Furthermore, NLRC5 gene expression decreased during smolt stage. These data suggest that NLRC5 participates in the Atlantic salmon immune response and is regulated, at least partly, by the smoltification process, suggesting that there is a depression of immune system from parr at smolt stage. This is the first report on the NLRC5 gene in salmonid smolts.
核苷酸结合寡聚化结构域样受体（NLRs）最近被鉴定为脊椎动物中的一种细胞内病原体识别受体家族。虽然NLRs在免疫系统中的参与情况已在各种哺乳动物模型中得到表征和分析，但很少有研究考虑硬骨鱼类中的NLRs。因此，本研究分析了大西洋鲑（Salmo salar）的NLRC5。在结构上，大西洋鲑NLRC5呈现富含亮氨酸重复序列的亚家族基因。在系统发育上，NLRC5在大西洋鲑和其他物种之间具有适度的保守性。实时定量PCR显示NLRC5在几乎所有分析的器官中均有表达，在头肾、脾脏和后肠中表达量最高。此外，NLRC5基因表达在银化阶段下降。这些数据表明NLRC5参与了大西洋鲑的免疫反应，并且至少部分受银化过程的调节，这表明在银化阶段幼鲑的免疫系统受到抑制。这是关于鲑科幼鱼NLRC5基因的首次报道。
