Kuruba B L, Buvani A P, Veluthambi K
Acta Virol. 2016;60(3):224-33. doi: 10.4149/av_2016_03_224.
Mungbean yellow mosaic virus-[India:Vigna] (MYMV-[IN:Vig]), a blackgram isolate of MYMV, causes yellow mosaic disease in blackgram and mungbean. Two variable DNA-B components, KA22 and KA27, cause distinct symptoms in blackgram [V. mungo (L.) Hepper] with the same DNA-A component. KA22 + DNA-A-agroinoculated blackgram plants displayed yellow mosaic symptom and accumulated high levels of viral single-stranded (ss) DNA. KA27 + DNA-A-agroinoculated blackgram plants displayed severe stunting symptom and accumulated very low levels of viral ssDNA. However, in mungbean [V. radiata (L.) Wilczek], KA27 + DNA-A caused yellow mosaic symptom and a high level of viral ssDNA accumulated. Swapping of KA27 DNA-B with the nuclear shuttle protein gene (NSP) of KA22 DNA-B (KA27xKA22 NSP) caused yellow mosaic symptom in blackgram, suggesting that KA22 NSP is the determinant of yellow mosaic symptom. Interestingly, KA27xKA22 NSP-infected blackgram plants accumulated high levels of viral ssDNA, comparable to that of KA22 DNA-B infection, suggesting that the KA22 NSP is responsible for accumulation of high levels of viral ssDNA. MYMV distribution was studied in blackgram and mungbean plants by leaf tissue hybridization, which showed mesophyll spread of the virus in KA22-infected blackgram leaflets and in KA27-infected mungbean leaflets, both of which displayed yellow mosaic symptom. However, the virus did not accumulate in the mesophyll in the case of KA27-infected blackgram leaflets. Interestingly, the swapped KA27xKA22 NSP-infected blackgram leaflets showed mesophyll accumulation of the virus, suggesting that KA22 NSP determines its mesophyll spread.
绿豆黄花叶病毒-[印度：豇豆]（MYMV-[IN：Vig]），一种MYMV的黑绿豆分离株，可在黑绿豆和绿豆中引发黄花叶病。两个可变的DNA-B组分，KA22和KA27，在具有相同DNA-A组分的黑绿豆[黑绿豆（V. mungo (L.) Hepper）]中会引发不同症状。KA22 + DNA-A农杆菌接种的黑绿豆植株表现出黄花叶症状，并积累了高水平的病毒单链（ss）DNA。KA27 + DNA-A农杆菌接种的黑绿豆植株表现出严重矮化症状，且病毒ssDNA积累水平极低。然而，在绿豆[绿豆（V. radiata (L.) Wilczek）]中，KA27 + DNA-A会引发黄花叶症状，并积累高水平的病毒ssDNA。将KA27的DNA-B与KA22的DNA-B的核穿梭蛋白基因（NSP）进行交换（KA27xKA22 NSP），会使黑绿豆出现黄花叶症状，这表明KA22 NSP是黄花叶症状的决定因素。有趣的是，KA27xKA22 NSP感染的黑绿豆植株积累了高水平的病毒ssDNA，与KA22 DNA-B感染的情况相当，这表明KA22 NSP负责高水平病毒ssDNA的积累。通过叶片组织杂交研究了MYMV在黑绿豆和绿豆植株中的分布，结果显示，在表现出黄花叶症状的KA22感染的黑绿豆小叶和KA27感染的绿豆小叶中，病毒在叶肉中扩散。然而，在KA27感染的黑绿豆小叶中，病毒并未在叶肉中积累。有趣的是，交换后的KA27xKA22 NSP感染的黑绿豆小叶显示病毒在叶肉中积累，这表明KA22 NSP决定了其在叶肉中的扩散。
