Kalyankumar Kamesh Krishnamoorthy, Malathi V G, Renukadevi P, S Mohan Kumar, Manivannan N, Patil S G, Karthikeyan G
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641003, India.
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641003, India.
Int J Biometeorol. 2022 Oct;66(10):1985-1995. doi: 10.1007/s00484-022-02334-x. Epub 2022 Aug 5.
The yellow mosaic disease (YMD) of blackgram caused by Mungbean yellow mosaic virus has emerged as a serious threat to grain legume production, especially in Southeastern Asia. Seasonal incidence of YMD with its vector population was assessed in three different agroclimatic zones of Tamil Nadu in India for three consecutive cropping seasons namely, Rabi 2018 (October-December), Summer 2019 (March-May), and Kharif 2019 (June-August) at three different time intervals viz., 20, 40, and 60 days after sowing (DAS). For all three seasons, disease incidence and whitefly count were recorded for a resistant and susceptible variety of blackgram in fields without any vector control intervention. The highest disease incidence (87%) was observed in the Panpozhi location during the summer season followed by Vamban and Coimbatore locations. The whitefly count was made through both visual count and yellow sticky traps. The whitefly population was highest at 20 DAS and decreased with the increasing age of crop for all the three locations assessed. Molecular epidemiology was analyzed by determining latent infection of mungbean yellow mosaic virus (MYMV) using molecular diagnosis. Latent infection was found to be well pronounced in the Coimbatore location during the Kharif season, where the crop was asymptomatic in both the resistant and susceptible varieties for all the three time periods assessed. The latent infection of MYMV observed in Coimbatore and Vamban ranged from 16.6 to 83.3% in both resistant and susceptible varieties for all three seasons. In Panpozhi, the latent infection of MYMV ranged from 16.6 to 66.6% for the susceptible variety (CO-5) for all three seasons observed. However, in the Panpozhi location, the resistant variety (VBN-8) failed to record any latent infection.
由绿豆黄花叶病毒引起的黑豆黄花叶病已成为豆类作物生产的严重威胁，特别是在东南亚地区。在印度泰米尔纳德邦的三个不同农业气候区，连续三个种植季节，即2018年冬季作物季（10月至12月）、2019年夏季作物季（3月至5月）和2019年季风作物季（6月至8月），于播种后三个不同时间间隔，即20天、40天和60天，评估了黄花叶病及其传毒介体种群的季节性发生情况。在所有三个季节中，在没有任何介体控制干预的田间，记录了一个抗性和一个感病黑豆品种的发病率和粉虱数量。在夏季，潘波齐地区观察到最高发病率（87%），其次是万班和哥印拜陀地区。粉虱数量通过目视计数和黄色粘虫板进行统计。在所评估的所有三个地点，粉虱种群在播种后20天最高，并随着作物年龄的增加而减少。通过分子诊断确定绿豆黄花叶病毒（MYMV）的潜伏感染，进行分子流行病学分析。发现在季风作物季哥印拜陀地区潜伏感染非常明显，在所评估的所有三个时间段，该地区抗性和感病品种的作物均无症状。在哥印拜陀和万班观察到的MYMV潜伏感染，在所有三个季节的抗性和感病品种中均为16.6%至83.3%。在潘波齐，在所观察的所有三个季节中，感病品种（CO-5）的MYMV潜伏感染率为16.6%至66.6%。然而，在潘波齐地区，抗性品种（VBN-8）未记录到任何潜伏感染。
