MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, P.R. China.
Sci Rep. 2016 Sep 19;6:33628. doi: 10.1038/srep33628.
The effects of cabbage waste (CW) addition on methane production in cow dung and corn straw co-fermentation systems were investigated. Four experimental groups, each containing 55 g of substrate, were set up as follows: 100% cow dung (C); 36% cabbage and 64% cow dung (CC); 36% straw and 64% cow dung (SC); and 18% cabbage, 18% straw, and 64% cow dung (CSC). After seven days of fermentation, the maximum methane yield was 134 mL in the CSC group, which was 2.81-fold, 1.78-fold, and 1340-fold higher than that obtained in the CC, SC, and C groups, respectively. CW treatment of the CSC group enhanced cellulase activity and enriched culturable cellulose-degrading bacterial strains. Miseq sequencing data revealed that the predominant phylum in the CSC group was Bacteroidetes, which contains most of the cellulose-degrading bacteria. Our results suggested that CW treatment elevated cellulose degradation and promoted methane production.
研究了添加甘蓝废弃物(CW)对牛粪和玉米秸秆共发酵系统中甲烷生成的影响。设置了四个实验组,每组包含 55g 基质,如下所示:100%牛粪(C);36%甘蓝和 64%牛粪(CC);36%秸秆和 64%牛粪(SC);18%甘蓝、18%秸秆和 64%牛粪(CSC)。发酵 7 天后,CSC 组的最大甲烷产量为 134mL,分别是 CC、SC 和 C 组的 2.81 倍、1.78 倍和 1340 倍。CSC 组中 CW 的处理提高了纤维素酶活性并富集了可培养的纤维素降解细菌菌株。Miseq 测序数据显示,CSC 组中的主要门是拟杆菌门,其中包含大多数纤维素降解细菌。我们的结果表明,CW 处理提高了纤维素降解并促进了甲烷生成。