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添加甘蓝促进纤维素降解提高牛粪与玉米秸秆共发酵产甲烷性能

Improving methane production in cow dung and corn straw co-fermentation systems via enhanced degradation of cellulose by cabbage addition.

机构信息

MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, P.R. China.

出版信息

Sci Rep. 2016 Sep 19;6:33628. doi: 10.1038/srep33628.

DOI:10.1038/srep33628
PMID:27641709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5027527/
Abstract

The effects of cabbage waste (CW) addition on methane production in cow dung and corn straw co-fermentation systems were investigated. Four experimental groups, each containing 55 g of substrate, were set up as follows: 100% cow dung (C); 36% cabbage and 64% cow dung (CC); 36% straw and 64% cow dung (SC); and 18% cabbage, 18% straw, and 64% cow dung (CSC). After seven days of fermentation, the maximum methane yield was 134 mL in the CSC group, which was 2.81-fold, 1.78-fold, and 1340-fold higher than that obtained in the CC, SC, and C groups, respectively. CW treatment of the CSC group enhanced cellulase activity and enriched culturable cellulose-degrading bacterial strains. Miseq sequencing data revealed that the predominant phylum in the CSC group was Bacteroidetes, which contains most of the cellulose-degrading bacteria. Our results suggested that CW treatment elevated cellulose degradation and promoted methane production.

摘要

研究了添加甘蓝废弃物(CW)对牛粪和玉米秸秆共发酵系统中甲烷生成的影响。设置了四个实验组,每组包含 55g 基质,如下所示:100%牛粪(C);36%甘蓝和 64%牛粪(CC);36%秸秆和 64%牛粪(SC);18%甘蓝、18%秸秆和 64%牛粪(CSC)。发酵 7 天后,CSC 组的最大甲烷产量为 134mL,分别是 CC、SC 和 C 组的 2.81 倍、1.78 倍和 1340 倍。CSC 组中 CW 的处理提高了纤维素酶活性并富集了可培养的纤维素降解细菌菌株。Miseq 测序数据显示,CSC 组中的主要门是拟杆菌门,其中包含大多数纤维素降解细菌。我们的结果表明,CW 处理提高了纤维素降解并促进了甲烷生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5c4409f40b02/srep33628-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/a2dab08cb6c2/srep33628-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5b22f86dc525/srep33628-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5f5f79147c45/srep33628-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/6257738141e0/srep33628-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5f010cfc2486/srep33628-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5c4409f40b02/srep33628-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/a2dab08cb6c2/srep33628-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5b22f86dc525/srep33628-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5f5f79147c45/srep33628-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/6257738141e0/srep33628-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5f010cfc2486/srep33628-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8896/5027527/5c4409f40b02/srep33628-f6.jpg

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