Tanaka Toyohito, Inomata Akiko
Division of Toxicology, Department of Pharmaceutical and Environmental Sciences, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Birth Defects Res B Dev Reprod Toxicol. 2016 Aug;107(4-5):195-205. doi: 10.1002/bdrb.21185. Epub 2016 Sep 19.
Female mice were exposed maternally to piperonyl butoxide (PBO) through diet to provide levels of 0 (control), 0.015, 0.03, and 0.06% during gestation and lactation periods, and selected reproductive and neurobehavioral parameters were measured in F generation. There was no adverse effect of PBO on litter size, litter weight, or sex ratio at birth. The average body weights of offspring showed no significant effects of PBO treatment through the lactation period in both sexes except for the low-dose group of females on PND 21. With respect to behavioral developmental parameters, swimming direction of female offspring on PND 7 was significantly accelerated in the low-dose group (p = 0.022). Exploratory behavior examination in male offspring indicated that total distance and movement time shortened significantly in dose-related manners (p = 0.0138 and 0.00231, respectively), average time of rearing lengthened significantly in a dose-related manner (p = 0.00814), and the frequencies of mice with urination was increased significantly in a dose-related manner (p < 0.05). For spontaneous behavior examination, the average time of movement in males and average time of rearing in females showed slightly dose-related effects in the F generation. The dose levels of PBO in the present study produced some adverse effects in neurobehavioral parameters in mice.
雌性小鼠在孕期和哺乳期通过饮食母体接触胡椒基丁醚（PBO），使其水平分别为0（对照）、0.015%、0.03%和0.06%，并在F代中测量选定的生殖和神经行为参数。PBO对产仔数、窝仔重或出生时的性别比例没有不良影响。除了在出生后第21天低剂量组的雌性后代外，在哺乳期，PBO处理对两性后代的平均体重均无显著影响。关于行为发育参数，低剂量组雌性后代在出生后第7天的游泳方向显著加快（p = 0.022）。雄性后代的探索行为检查表明，总距离和移动时间以剂量相关的方式显著缩短（分别为p = 0.0138和0.00231），直立平均时间以剂量相关的方式显著延长（p = 0.00814），排尿小鼠的频率以剂量相关的方式显著增加（p < 0.05）。对于自发行为检查，在F代中，雄性的平均移动时间和雌性的平均直立时间显示出轻微的剂量相关效应。本研究中PBO的剂量水平对小鼠的神经行为参数产生了一些不良影响。
