GIMAP-EA3064, University of Lyon, Saint-Etienne, France.
Auvergne-Loire French Blood Establishment, Saint-Etienne, France.
Blood Transfus. 2018 Jan;16(1):63-72. doi: 10.2450/2016.0076-16. Epub 2016 Sep 8.
Leucoreduction of blood components, including platelet components, is strongly encouraged but not yet universal, especially outside high income countries. As both leucocytes and platelets secrete copious amounts of pro-inflammatory cytokines/chemokines under various conditions and during storage, we investigated the potential of the respective secretory programmes of these cells in order to evaluate their subsequent pathophysiological effects.
A total of 158 individual non-leucoreduced platelet components were obtained from Tunisian donors and tested for characteristic biological response modifiers (BRM) of leukocytes (IL-1β, IL-8), platelets (sCD62P, sCD40L) and both cell types (TNF-α, RANTES) in the presence or absence of thrombin stimulation and after different periods of storage (up to 5 days). BRM levels were determined using enzyme-linked immunosorbent assays and Luminex technology. Platelet-leucocyte aggregate formation during storage was analysed using flow cytometry.
Leucocyte- and platelet-associated BRM had clearly distinct profiles both at the onset (day 0) and termination (day 5) of the observation period but altered during the intermediate period so that their respective importance was inverted; in fact, the profiles were merged and indistinguishable on days 2-3. The leucocyte-derived BRM largely dominated over platelet-derived ones and further altered the BRM platelet secretion programme.
This study contributes substantial, new information on leucocyte/platelet interactions and their likely role in transfusion when leucodepletion cannot be performed or is only partially achieved.
血液成分（包括血小板成分）的白细胞减少法受到强烈鼓励，但尚未普及，尤其是在高收入国家之外。由于白细胞和血小板在各种条件下和储存期间都会大量分泌促炎细胞因子/趋化因子，我们研究了这些细胞各自分泌程序的潜力，以便评估它们随后的病理生理效应。
从突尼斯供体中获得了总共 158 份未经过白细胞减少法处理的血小板成分，并在存在或不存在凝血酶刺激以及不同储存期（最长 5 天）的情况下，对其进行了白细胞（IL-1β、IL-8）、血小板（sCD62P、sCD40L）和两种细胞类型（TNF-α、RANTES）的特征性生物反应调节剂（BRM）的检测。BRM 水平使用酶联免疫吸附测定法和 Luminex 技术进行测定。使用流式细胞术分析储存过程中血小板-白细胞聚集体的形成。
白细胞和血小板相关的 BRM 在观察期开始（第 0 天）和结束（第 5 天）时都有明显的差异，但在中间期会发生改变，因此它们各自的重要性会颠倒；事实上，在第 2-3 天，这些特征会融合并且难以区分。白细胞衍生的 BRM 大大超过了血小板衍生的 BRM，并进一步改变了 BRM 血小板分泌程序。
本研究提供了关于白细胞/血小板相互作用及其在无法进行或仅部分实现白细胞减少法时在输血中可能作用的大量新信息。
