Leibniz-Institute of Photonic Technology Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany.
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany.
Sci Rep. 2016 Sep 20;6:33547. doi: 10.1038/srep33547.
An in cellulo study of the ultrafast excited state processes in the paradigm molecular light switch Ru(bpy)2dppz by localized pump-probe spectroscopy is reported for the first time. The localization of Ru(bpy)2dppz in HepG2 cells is verified by emission microscopy and the characteristic photoinduced picosecond dynamics of the molecular light switch is observed in cellulo. The observation of the typical phosphorescence stemming from a (3)MLCT state suggests that the Ru(bpy)2dppz complex intercalates with the DNA in the nucleus. The results presented for this benchmark coordination compound reveal the necessity to study the photoinduced processes in coordination compounds for intracellular use, e.g. as sensors or as photodrugs, in the actual biological target environment in order to derive a detailed molecular mechanistic understanding of the excited-state properties of the systems in the actual biological target environment.
首次通过局域泵浦探针光谱法在细胞内研究了典范分子光开关 Ru(bpy)2dppz 的超快激发态过程。通过发射显微镜验证了 Ru(bpy)2dppz 在 HepG2 细胞中的定位，并在细胞内观察到分子光开关的特征光诱导皮秒动力学。观察到源自 (3)MLCT 态的典型磷光表明 Ru(bpy)2dppz 配合物与核内的 DNA 相互插入。为该基准配合物呈现的结果表明，有必要在实际的生物靶环境中研究用于细胞内使用的配合物的光诱导过程，例如作为传感器或光药物，以便在实际的生物靶环境中获得对系统激发态性质的详细分子机制理解。
