Gorukmez Ozlem, Yakut Tahsin, Gorukmez Orhan, Sag Sebnem Ozemri, Topak Ali, Sahinturk Serdar, Kanat Ozkan
Uludag University, School of Medicine, Department of Medical Genetics, Bursa, Turkey E-mail :
Asian Pac J Cancer Prev. 2016;17(8):3855-9.
Colorectal cancer (CRC) is reported to be the third most common cancer worldwide and the fourth most common cause of cancer related deaths. CRC is considered to be a multifactorial disease whose risk varies due to the complex interaction between individual genetic basis and exposure to multiple endogenous factors. Glutathione S-transferases are pro-carcinogenic in CRC and are required for the conjugation between chemotherapeutics and broad spectrum xenobiotics. One hundred and eleven patients with CRC and 128 control subjects without any cancer history were enrolled in this study. Multiplex PCR was applied to determine polymorphisms for the GSTT1 and M1 genes, and PCR-RFLP was applied for the GSTP1 (Ile105Val) gene polymorphism. Values <0.05 were defined as statistically significant. We detected a significant high correlation between predisposition for CRC and presence of the Ile/Ile genotype of the GSTP1 (IIe105Val) gene polymorphism, but we did not find a significant relationship between predisposition for CRC and GSTT1 and M1 deletion polymorphisms. In addition, we did not determine a relationship between GSTT1, M1 and P1 gene polymorphisms and any clinicopathological features of CRC. GSTT1 null/GSTM1 positive and GSTT1 null/GSTM1 positive/GSTP1 Ile/ Ile genotypes were significantly higher in the patient group. Our results revealed that there is no relationship among CRC, its clinicopathologic features, and GSTT1 M1 gene polymorphisms. However, there was a significant correlation between CRC and the GSTP1 Ile/Ile genotype. Further studies with larger patient groups are required to delineate the relationships between GST gene polymorphisms and the clinicopathologic features of CRC in Turkey.
据报道，结直肠癌（CRC）是全球第三大常见癌症，也是癌症相关死亡的第四大常见原因。CRC被认为是一种多因素疾病，由于个体遗传基础与多种内源性因素暴露之间的复杂相互作用，其风险各不相同。谷胱甘肽S-转移酶在CRC中具有促癌作用，是化疗药物与广谱外源性物质结合所必需的。本研究纳入了111例CRC患者和128例无任何癌症病史的对照受试者。采用多重PCR检测GSTT1和M1基因的多态性，采用PCR-RFLP检测GSTP1（Ile105Val）基因多态性。P值<0.05被定义为具有统计学意义。我们检测到CRC易感性与GSTP1（IIe105Val）基因多态性的Ile/Ile基因型之间存在显著的高度相关性，但未发现CRC易感性与GSTT1和M1缺失多态性之间存在显著关系。此外，我们未确定GSTT1、M1和P1基因多态性与CRC的任何临床病理特征之间的关系。患者组中GSTT1缺失/GSTM1阳性和GSTT1缺失/GSTM1阳性/GSTP1 Ile/Ile基因型显著更高。我们的结果显示，CRC与其临床病理特征以及GSTT1 M1基因多态性之间没有关系。然而，CRC与GSTP1 Ile/Ile基因型之间存在显著相关性。需要对更大的患者群体进行进一步研究，以阐明土耳其GST基因多态性与CRC临床病理特征之间的关系。
