Eroğlu Pınar, Erkol İnal Esra, Sağ Şebnem Özemri, Görükmez Özlem, Topak Ali, Yakut Tahsin
Department of Physical Medicine and Rehabilitation, Ankara Occupational Disease Hospital, Ankara, Turkey.
Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Süleyman Demirel University, Çünür, Isparta, Turkey.
Clin Rheumatol. 2016 May;35(5):1245-51. doi: 10.1007/s10067-014-2855-0. Epub 2015 Jan 9.
Oxidative stress was related with carpal tunnel syndrome (CTS). We aimed to clarify the associations between glutathione S-transferase (GST)M1, GSTT1 and GSTP1-Ile105Val polymorphisms and CTS. One hundred-forty patients with CTS and 97 healthy controls were enrolled in this study. Tinel and Phalen signs were noted as positive or negative. Functional and clinical status of patients was evaluated by the Boston Questionnaire. The intensity of hand and/or wrist pain was evaluated on 10 cm visual analog scale (VAS). We applied the polymerase chain reaction (PCR) to determine the polymorphisms of the GSTM1 and GSTT1 and the PCR-restriction fragment length polymorphism method for detecting the GSTP1-Ile105Val polymorphism. The M1 null genotype was significantly higher in patients with CTS compared to healthy controls, and the M1 null genotype seemed to increase the risk of CTS approximately two-fold (P = 0.011; odds ratio (OR) = 1.98; 95 % confidence interval (CI) 1.17-3.36). The M1 null, T1 present combined genotype was significantly higher in patients with CTS compared to healthy controls (P = 0.043); however, it seemed not to increase the risk of CTS (P = 0.14; OR = 0.62; 95 % CI 0.33-1.76). We found significantly higher levels of the VAS, Boston Symptom Severity Scale and Phalen sign in patients with the Ile/Val or the Val/Val genotypes compared to those in patients with the Ile/Ile genotype (P = 0.003, 0.004 and 0.044, respectively). We proposed that genes involved in the protection from oxidative stress may influence the susceptibility, clinical and functional status of CTS. The GSTM1 null genotype may be related with the development of CTS, whereas the Val allele of GSTP1-Ile105Val polymorphism may be associated with worse functional and clinical status in CTS.
氧化应激与腕管综合征（CTS）相关。我们旨在阐明谷胱甘肽S-转移酶（GST）M1、GSTT1和GSTP1-Ile105Val基因多态性与CTS之间的关联。本研究纳入了140例CTS患者和97例健康对照。Tinel征和Phalen征记录为阳性或阴性。通过波士顿问卷评估患者的功能和临床状态。采用10厘米视觉模拟量表（VAS）评估手和/或腕部疼痛的强度。我们应用聚合酶链反应（PCR）来确定GSTM1和GSTT1的基因多态性，并采用PCR-限制性片段长度多态性方法检测GSTP1-Ile105Val基因多态性。与健康对照相比，CTS患者中M1无效基因型显著更高，且M1无效基因型似乎使CTS风险增加约两倍（P = 0.011；比值比（OR）= 1.98；95%置信区间（CI）1.17 - 3.36）。与健康对照相比，CTS患者中M1无效、T1存在的联合基因型显著更高（P = 0.043）；然而，它似乎并未增加CTS风险（P = 0.14；OR = 0.62；95% CI 0.33 - 1.76）。我们发现，与Ile/Ile基因型患者相比，Ile/Val或Val/Val基因型患者的VAS、波士顿症状严重程度量表和Phalen征水平显著更高（分别为P = 0.003、0.004和0.044）。我们提出，参与抗氧化应激保护的基因可能影响CTS的易感性、临床和功能状态。GSTM1无效基因型可能与CTS的发生有关，而GSTP1-Ile105Val基因多态性的Val等位基因可能与CTS中更差的功能和临床状态相关。
