Reader S C, Davison B, Robertson W R, Lambert A
Department of Chemical Pathology, Hope Hospital, Salford, UK.
Ann Clin Biochem. 1989 Jul;26 ( Pt 4):341-5. doi: 10.1177/000456328902600408.
The biopotency of six preparations of thyrotrophin (TSH) has been compared in a highly sensitive in vitro porcine thyroid cell bioassay using iodide uptake as an endpoint. Three of these preparations were of human origin and three derived from bovine pituitary tissue. One human TSH preparation, the 2nd International Reference Preparation, 80/558, was used to calibrate the other five. The log dose-log response curves for all preparations were sigmoidal in shape. For the purpose of evaluation the central linear portions of the curves were compared. With all preparations the slopes in this region were very similar. The relative biopotencies of the bovine preparations (unit:unit) were at least five times those of the human standards when measured using the porcine thyroid cell bioassay. These findings emphasise the need to control the TSH standards employed in a variety of bioassays, particularly those used for between-laboratory comparison.
已在一项高度灵敏的体外猪甲状腺细胞生物测定中，以碘摄取作为终点，比较了六种促甲状腺激素（TSH）制剂的生物效价。其中三种制剂源自人，三种源自牛垂体组织。一种人TSH制剂，即第二国际参考制剂80/558，用于校准其他五种制剂。所有制剂的对数剂量-对数反应曲线均呈S形。为进行评估，比较了曲线的中心线性部分。所有制剂在该区域的斜率非常相似。当使用猪甲状腺细胞生物测定法测量时，牛制剂的相对生物效价（单位：单位）至少是人类标准制剂的五倍。这些发现强调了控制各种生物测定中所用TSH标准的必要性，尤其是那些用于实验室间比较的标准。
