Miyagi Masaru, Kasumov Takhar
Center for Proteomics and Bioinformatics, Department of Nutrition, Case Western Reserve University, Cleveland, OH 44106, USA
Mass Spectrometry Laboratory, Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH 44272, USA.
Philos Trans A Math Phys Eng Sci. 2016 Oct 28;374(2079). doi: 10.1098/rsta.2015.0378.
The controlled and selective synthesis/clearance of biomolecules is critical for most cellular processes. In most high-throughput 'omics' studies, we measure the static quantities of only one class of biomolecules (e.g. DNA, mRNA, proteins or metabolites). It is, however, important to recognize that biological systems are highly dynamic in which biomolecules are continuously renewed and different classes of biomolecules interact and affect each other's production/clearance. Therefore, it is necessary to measure the turnover of diverse classes of biomolecules to understand the dynamic nature of biological systems. Herein, we explain why the kinetic analysis of a diverse range of biomolecules is important and how such an analysis can be done. We argue that heavy water ((2)H2O) could be a universal tracer for monitoring the synthesis of biomolecules on a global scale.This article is part of the themed issue 'Quantitative mass spectrometry'.
生物分子的可控和选择性合成/清除对于大多数细胞过程至关重要。在大多数高通量“组学”研究中，我们仅测量一类生物分子（例如DNA、mRNA、蛋白质或代谢物）的静态量。然而，必须认识到生物系统是高度动态的，其中生物分子不断更新，不同类别的生物分子相互作用并影响彼此的产生/清除。因此，有必要测量不同类别的生物分子的周转率，以了解生物系统的动态性质。在此，我们解释了为什么对多种生物分子进行动力学分析很重要以及如何进行这种分析。我们认为重水（(2)H2O）可能是用于在全球范围内监测生物分子合成的通用示踪剂。本文是主题为“定量质谱”的特刊的一部分。
