Stoica G, O'Leary M
Department of Veterinary Pathology, Texas A&M University, College Station 77840.
Anticancer Res. 1989 May-Jun;9(3):679-85.
A panel of seven fluorescein isothiocyanate (FITC) labeled lectins were used to study the distribution of specific binding sites in histologic sections of rat ovaries and ENU-induced Sertoli cell tumors (SCT) of the ovaries. Ten SCT and 5 normal ovaries derived from Berlin Druckey IV (BD-IV) rats were examined by FITC lectins. The tissues examined were fixed in 10% buffered formalin and embedded in paraffin blocks. In normal ovaries, lectin binding sites were more uniform, ordered and consistent than in ovarian SCT where some lectin staining appeared disorderly inconsistent and varied with the degree of tumor differentiation. Two lectins, (from Triticum vulgaris [WGA] and Arachis hypogaea [PNA], uniformly stained the apices of the ovarian surface epithelium and subadjacent tunica vaginalis. The ovarian stroma, oocyte nucleus, follicular and granulosa-theca cells, stained uniformly strong with succinated Con A (from Con-canavalia ensiformis). Three lectins (from Triticum vulgaris, Ulex europeaus [UEA-1] and Arachis hypogeae) accentuated the basal lamina in the SCT and normal ovarian follicles. The zona pellucida was strongly labeled with lectin derived from Triticum vulgaris, Ricinus communis (RCA) and moderately with lectin derived from Arachis hypogeae. The oviduct ampulla exhibited an intracytoplasmic strong vesicular labeling with lectins derived from Triticum vulgare, Dolichos biflorus (DBA), Glycin max (Soybean-SBA) and Arachis hypogeae. The SCT cells showed an inconsistent, irregular labeling pattern with lectins derived from Ulex europaeus, Dolichos biflorus and Soybean mostly as a coarse granular cytoplasmic labeling. Neuraminidase digestion enhanced lectin staining with PNA in normal ovary and in SCT. This data provided at list of lectin markers for distinct components of the BD-IV rat ovary and ovarian SCT.
使用一组七种异硫氰酸荧光素（FITC）标记的凝集素来研究大鼠卵巢组织切片和ENU诱导的卵巢支持细胞瘤（SCT）中特异性结合位点的分布。通过FITC凝集素检查了来自柏林德鲁基IV（BD-IV）大鼠的10个SCT和5个正常卵巢。检查的组织用10%缓冲福尔马林固定并包埋在石蜡块中。在正常卵巢中，凝集素结合位点比卵巢SCT中更均匀、有序和一致，在卵巢SCT中，一些凝集素染色显得无序、不一致且随肿瘤分化程度而变化。两种凝集素（来自普通小麦[WGA]和花生[PNA]）均匀地染色卵巢表面上皮的顶端和相邻的阴道膜。卵巢基质、卵母细胞核、卵泡和颗粒-膜细胞，用琥珀酰化刀豆球蛋白A（来自刀豆）均匀强烈染色。三种凝集素（来自普通小麦、欧洲荆豆[UEA-1]和花生）突出了SCT和正常卵巢卵泡中的基底层。透明带被来自普通小麦、蓖麻（RCA）的凝集素强烈标记，被来自花生的凝集素中度标记。输卵管壶腹用来自普通小麦、双花扁豆（DBA）、大豆（大豆-SBA）和花生的凝集素表现出胞浆内强烈的囊泡状标记。SCT细胞对来自欧洲荆豆、双花扁豆和大豆的凝集素显示出不一致、不规则的标记模式，主要为粗颗粒状胞浆标记。神经氨酸酶消化增强了正常卵巢和SCT中PNA的凝集素染色。该数据提供了BD-IV大鼠卵巢和卵巢SCT不同成分的凝集素标记列表。
