Söderström K O
Department of Pathology, University of Turku, Finland.
Anat Rec. 1988 Feb;220(2):219-23. doi: 10.1002/ar.1092200214.
Formalin-fixed, paraffin-embedded tissue sections of the human retina were stained with different fluorescein isothiocyanate-conjugated lectins. The lectins used were concanavalin A (Con A), Triticum vulgaris (WGA), glycine maximum (SBA), Dolichos biflorus (DBA), Ulex europaeus (UEA I), Arachis hypogaea (PNA), and Ricinus communis (RCA I). Con A stained both the inner and outer segments of the rods and cones, whereas WGA stained the inner and outer segments of the rods and the outer segments of the cones. PNA selectively stained only the inner segments of the cones. In addition, Con A and WGA stained neuron cytoplasm and nerve fibers in different layers of the retina. The results obtained differ in some important aspects from those previously obtained in the frog and monkey retina; this finding may be due to species differences. The results of lectin staining in the normal human retina may form the basis for future studies of retinal diseases.
人视网膜的福尔马林固定石蜡包埋组织切片用不同的异硫氰酸荧光素偶联凝集素进行染色。所用的凝集素有刀豆球蛋白A（Con A）、小麦胚凝集素（WGA）、大豆凝集素（SBA）、双花扁豆凝集素（DBA）、荆豆凝集素（UEA I）、花生凝集素（PNA）和蓖麻凝集素（RCA I）。Con A对视杆细胞和视锥细胞的内节和外节均有染色，而WGA对视杆细胞的内节和外节以及视锥细胞的外节有染色。PNA仅选择性地对视锥细胞的内节进行染色。此外，Con A和WGA对视网膜不同层的神经元细胞质和神经纤维进行染色。所获得的结果在一些重要方面与先前在青蛙和猴子视网膜中获得的结果不同；这一发现可能是由于物种差异。正常人类视网膜中凝集素染色的结果可能为未来视网膜疾病的研究奠定基础。
