Gao Hanjing, Song Qing, Lv Faqin, Wang Shan, Wang Yiru, Li Xiaoyan, Luo Yukun, Mei Xingguo, Tang Jie
Department of Ultrasound, Chinese PLA General Hospital, Beijing, China.
Department of Ultrasound, 161th Hospital of Chinese PLA, Wuhan, China.
Gut Liver. 2017 Jan 15;11(1):156-163. doi: 10.5009/gnl15281.
BACKGROUND/AIMS: This study investigated the protection provided by gabexate mesylate thermo-sensitive gel (GMTI) against grade III pancreatic trauma in rats.
A grade III pancreatic trauma model with main pancreatic duct dividing was established, and the pancreas anatomical diagram, ascites, and serum biochemical indices, including amylase, lipase, C-reactive protein (CRP), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α), were examined. The pancreas was sliced and stained with hematoxylin eosin and subjected to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.
Ascites, serum amylase, lipase, CRP, IL-6, and TNF-α levels were significantly increased in the pancreas trauma (PT) groups with prolonged trauma time and were significantly decreased after GMTI treatment. The morphological structure of the pancreas was loose, the acinus was significantly damaged, the nuclei were irregular and hyperchromatic, and there was inflammatory cell invasion in the PT group compared to the control. After GMTI treatment, the morphological structure of the pancreas was restored, and the damaged acinus and inflammatory cell invasion were decreased compared to the PT group. Moreover, the cell apoptosis index was significantly increased in the PT group and restored to the same levels as the control group after GMTI treatment.
GMTI, a novel formulation and drug delivery method, exhibited specific effective protection against PT with acute pancreatitis therapy and has potential value as a minimally invasive adjuvant therapy for PT with acute pancreatitis.
背景/目的：本研究探讨甲磺酸加贝酯热敏凝胶（GMTI）对大鼠Ⅲ级胰腺创伤的保护作用。
建立主胰管断裂的Ⅲ级胰腺创伤模型，检测胰腺解剖图、腹水及血清生化指标，包括淀粉酶、脂肪酶、C反应蛋白（CRP）、白细胞介素6（IL-6）和肿瘤坏死因子-α（TNF-α）。将胰腺切片，进行苏木精-伊红染色及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）染色。
随着创伤时间延长，胰腺创伤（PT）组腹水、血清淀粉酶、脂肪酶、CRP、IL-6和TNF-α水平显著升高，GMTI治疗后显著降低。与对照组相比，PT组胰腺形态结构疏松，腺泡明显受损，细胞核不规则且染色质增多，有炎性细胞浸润。GMTI治疗后，胰腺形态结构恢复，与PT组相比，受损腺泡及炎性细胞浸润减少。此外，PT组细胞凋亡指数显著升高，GMTI治疗后恢复至与对照组相同水平。
GMTI作为一种新型制剂和给药方法，在急性胰腺炎治疗中对PT表现出特异性有效保护作用，作为PT合并急性胰腺炎的微创辅助治疗具有潜在价值。
