Owens Sean D, Kol Amir, Walker Naomi J, Borjesson Dori L
Department of Pathology, Microbiology, Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Stem Cells Int. 2016;2016:5830103. doi: 10.1155/2016/5830103. Epub 2016 Aug 28.
Background. It is unknown whether horses that receive allogeneic mesenchymal stem cells (MSCs) injections develop specific humoral immune response. Our goal was to develop and validate a flow cytometric MSC crossmatch procedure and to determine if horses that received allogeneic MSCs in a clinical setting developed measurable antibodies following MSC administration. Methods. Serum was collected from a total of 19 horses enrolled in 3 different research projects. Horses in the 3 studies all received unmatched allogeneic MSCs. Bone marrow (BM) or adipose tissue derived MSCs (ad-MSCs) were administered via intravenous, intra-arterial, intratendon, or intraocular routes. Anti-MSCs and anti-bovine serum albumin antibodies were detected via flow cytometry and ELISA, respectively. Results. Overall, anti-MSC antibodies were detected in 37% of the horses. The majority of horses (89%) were positive for anti-bovine serum albumin (BSA) antibodies prior to and after MSC injection. Finally, there was no correlation between the amount of anti-BSA antibody and the development of anti-MSC antibodies. Conclusion. Anti allo-MSC antibody development was common; however, the significance of these antibodies is unknown. There was no correlation between either the presence or absence of antibodies and the percent antibody binding to MSCs and any adverse reaction to a MSC injection.
背景。接受同种异体间充质干细胞（MSC）注射的马匹是否会产生特异性体液免疫反应尚不清楚。我们的目标是开发并验证一种流式细胞术MSC交叉配型程序，并确定在临床环境中接受同种异体MSC的马匹在注射MSC后是否会产生可检测到的抗体。方法。从参与3个不同研究项目的总共19匹马中采集血清。这3项研究中的马匹均接受了不匹配的同种异体MSC。通过静脉内、动脉内、肌腱内或眼内途径给予骨髓（BM）或脂肪组织来源的MSC（ad-MSC）。分别通过流式细胞术和ELISA检测抗MSC抗体和抗牛血清白蛋白抗体。结果。总体而言，37%的马匹检测到抗MSC抗体。大多数马匹（89%）在注射MSC之前和之后抗牛血清白蛋白（BSA）抗体均呈阳性。最后，抗BSA抗体的量与抗MSC抗体的产生之间没有相关性。结论。抗同种异体MSC抗体的产生很常见；然而，这些抗体的意义尚不清楚。抗体的存在与否以及抗体与MSC结合的百分比和对MSC注射的任何不良反应之间均无相关性。
