Liu D-C, Yang X-N, Huang C-Z, Sun Y-G, Dai X-M
Department of Orthopaedics, the First Hospital of Xuzhou, Xuzhou, China.
Eur Rev Med Pharmacol Sci. 2016 Sep;20(17):3535-43.
To observe whether adipose-derived stem cells (ADSCS), co-cultured with osteoblasts, can differentiate into osteoblasts and, if so, to study the best-induced conditions, with an ultimate goal of repairing bone defects.
Adipose-derived stem cells and osteoblasts were isolated from New Zealand white rabbits, and co-cultured in media with either 5% or 10% fetal bovine serum, for up to 4 weeks. The morphology of collected cells was examined under a microscope, and histological staining with alkaline phosphatase and alizarin red was carried out after induction for 1, 2, 3 and 4 weeks. Osteogenesis identification, including mRNA expression of type I collagen and osteocalcin, and alkaline phosphatase, was also performed using RT-PCR.
After 7 days of co-culture, some adipose-derived stem cells became round in both groups. After 14 days of co-culture, adipose-derived stem cells were found highly-differentiated, and stained positively with alkaline phosphatase and alizarin red, similar to mature osteoblasts. The mRNA expression of type I collagen and osteocalcin increased in both groups, especially in the 10% fetal bovine serum group.
Our findings indicate that adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts when induced by a high concentration of serum culture.
观察脂肪来源干细胞（ADSCS）与成骨细胞共培养时能否分化为成骨细胞，若能分化，则研究最佳诱导条件，最终目标是修复骨缺损。
从新西兰白兔中分离脂肪来源干细胞和成骨细胞，并在含有5%或10%胎牛血清的培养基中共培养长达4周。在显微镜下检查收集细胞的形态，并在诱导1、2、3和4周后进行碱性磷酸酶和茜素红组织学染色。还使用逆转录聚合酶链反应（RT-PCR）进行成骨鉴定，包括I型胶原蛋白、骨钙素和碱性磷酸酶的mRNA表达。
共培养7天后，两组中的一些脂肪来源干细胞都变成了圆形。共培养14天后，发现脂肪来源干细胞高度分化，并用碱性磷酸酶和茜素红染色呈阳性，类似于成熟成骨细胞。两组中I型胶原蛋白和骨钙素的mRNA表达均增加，尤其是在10%胎牛血清组中。
我们的研究结果表明，与成骨细胞共培养的脂肪来源干细胞在高浓度血清培养诱导下可分化为成骨细胞。
