State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
Sci Rep. 2017 Jan 5;7:40088. doi: 10.1038/srep40088.
During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface. Importantly, the compound CAs of PDLSCs and JBMSCs regenerated periodontal ligament-like fibers and mineralized matrix on the Ti scaffold surface, both in nude mice ectopic and minipig orthotopic transplantations. Our data revealed that an effective regenerative microenvironment, reconstructed by JBMSCs, promoted periodontium regeneration by regulating PDLSCs function on the Ti material.
在牙齿发育过程中，颌骨与牙胚相互作用，为其提供发育的微环境。颌骨来源的间充质干细胞（JBMSCs）维持着这种微环境，以促进牙根和牙周组织的发育。然而，颌骨微环境对牙周组织再生的影响在很大程度上仍是难以捉摸的。我们之前的研究表明，骨髓间充质干细胞的细胞聚集体（CAs）促进了处理后的牙本质支架上牙周组织的再生。在这里，我们发现 JBMSCs 不仅增强了牙周膜干细胞（PDLSCs）的成骨分化，还增强了它们在钛（Ti）材料表面的黏附性。重要的是，PDLSCs 和 JBMSCs 的复合 CAs 在裸鼠异位和小型猪原位移植中，在 Ti 支架表面再生了牙周韧带样纤维和矿化基质。我们的数据揭示了由 JBMSCs 重建的有效再生微环境，通过调节 PDLSCs 在 Ti 材料上的功能，促进了牙周组织的再生。
