Barkley D, Allard S, Feldmann M, Maini R N
Division of Clinical Immunology, Kennedy Institute of Rheumatology, London, United Kingdom.
Arthritis Rheum. 1989 Aug;32(8):955-63. doi: 10.1002/anr.1780320804.
We investigated cellular phenotypes and expression of class II major histocompatibility complex antigens on endothelium and cellular infiltrates in synovium from patients with rheumatoid arthritis (RA) or reactive arthritis, using an indirect immunoperoxidase technique. The RA specimens showed synovial lining layer hypertrophy and several focal accumulations of lymphocytes, both of which were absent in the reactive arthritis synovium. The percentage of cells expressing monocyte/macrophage markers was significantly higher in RA specimens. The percentages of cells expressing B and T cell markers were similar in both diseases. There was no significant difference in the expression of HLA-DR or DP by endothelium in the 2 diseases, but a marked increase in expression of HLA-DQ by endothelium was observed in the RA synovium versus that from patients with reactive arthritis. This overexpression of HLA-DQ was also seen in the interstitial cells of RA patients compared with reactive arthritis patients. In the reactive arthritis synovium, a significant population of cells (30%) was noted to be HLA-DR positive, and negative for macrophage and lymphocyte markers. Some of these cells had a dendritic morphology. The coexpression of HLA-DQ and HLA-DR may play an important role in antigen presentation and disease chronicity in RA.
我们采用间接免疫过氧化物酶技术，研究了类风湿性关节炎（RA）或反应性关节炎患者滑膜中内皮细胞的细胞表型、II类主要组织相容性复合体抗原的表达以及细胞浸润情况。RA标本显示滑膜衬里层肥大以及淋巴细胞的多处局灶性聚集，而这些在反应性关节炎滑膜中均不存在。RA标本中表达单核细胞/巨噬细胞标志物的细胞百分比显著更高。两种疾病中表达B和T细胞标志物的细胞百分比相似。两种疾病中内皮细胞HLA - DR或DP的表达无显著差异，但与反应性关节炎患者相比，RA滑膜中内皮细胞HLA - DQ的表达明显增加。与反应性关节炎患者相比，RA患者的间质细胞中也可见HLA - DQ的这种过表达。在反应性关节炎滑膜中，有相当数量的细胞（30%）被发现HLA - DR呈阳性，而巨噬细胞和淋巴细胞标志物呈阴性。其中一些细胞具有树突状形态。HLA - DQ和HLA - DR的共表达可能在RA的抗原呈递和疾病慢性化中起重要作用。
