Siddique Mam, Linhart O, Kujawa R, Krejszeff S, Butts Iae
Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic.
Department of Lake and River Fisheries, Warmia and Mazury University, Olsztyn, Poland.
Reprod Domest Anim. 2016 Dec;51(6):960-969. doi: 10.1111/rda.12773. Epub 2016 Sep 21.
Seminal plasma (SP) and ovarian fluid (OF) plays an important role as storage media to prevent the activation of gametes both in vivo and under artificial conditions. The objectives of this study were to quantify gamete biochemistry and explore correlations among quantitative characteristics of SP, OF and sperm performance traits of Ide Leuciscus idus and Northern pike Esox lucius. Generally, Na , K and Cl were found to be the most dominating ions, although concentrations of K were higher in SP, while Na and Cl concentrations were higher in OF for both species. Several significant correlations among the biochemical properties such as total protein, glucose, osmolality, cholesterol, K , Ca , Cl and Mg were observed for SP and OF. Total protein content of Ide SP was positively correlated with sperm activity traits (r ≥ .89, p ≤ .05), while K concentration was negatively correlated with sperm traits (r ≥ -.89, p ≤ .05). Moreover, Ca concentration in Northern pike SP was positively correlated with the percentage of sperm motility (r = . 98, p < .01). In conclusion, these results can be used to better understand the biochemistry of SP and OF, improve methods for short- and long-term storage of gametes and standardize fertilization protocols.
精浆（SP）和卵巢液（OF）作为储存介质，在体内和人工条件下防止配子激活方面发挥着重要作用。本研究的目的是量化配子生物化学，并探索白斑狗鱼和白斑狗鱼的精浆、卵巢液的定量特征与精子性能特征之间的相关性。一般来说，钠、钾和氯被发现是最主要的离子，尽管两种鱼的精浆中钾的浓度较高，而卵巢液中钠和氯的浓度较高。在精浆和卵巢液中，观察到总蛋白、葡萄糖、渗透压、胆固醇、钾、钙、氯和镁等生化特性之间存在若干显著相关性。白斑狗鱼精浆的总蛋白含量与精子活力特征呈正相关（r≥0.89，p≤0.05），而钾浓度与精子特征呈负相关（r≥-0.89，p≤0.05）。此外，白斑狗鱼精浆中的钙浓度与精子活力百分比呈正相关（r = 0.98，p < 0.01）。总之，这些结果可用于更好地理解精浆和卵巢液的生物化学，改进配子短期和长期储存方法，并规范受精方案。
