Cejko Beata Irena, Krejszeff Sławomir, Cejko Agata Anna, Dryl Katarzyna
Department of Salmonid Research, Stanisław Sakowicz Inland Fisheries Institute, 10-719 Olsztyn, Poland.
Department of Aquaculture, Stanisław Sakowicz Inland Fisheries Institute, 10-917 Olsztyn, Poland.
Animals (Basel). 2023 Feb 6;13(4):572. doi: 10.3390/ani13040572.
The purpose of this study was to compare four activation solutions (AS)-Woynarovich, Lahnsteiner, Kucharczyk, and Perchec-with the addition of 0.5% bovine serum albumin (BSA) for ide () sperm activation and analysis with a CASA system. It was found that ide sperm can be activated using each AS within a pH range of 7.4-9.0 and an osmolality range of 160-200 mOsm kg. The effect of Woynarovich and Perchec solutions supplemented with BSA and casein at concentrations of 0.25, 0.5, 1.0, and 2.0% were also analyzed during the experiment. These two AS without protein supplementation (pure solutions) were the controls. Woynarovich and Perchec solutions supplemented with the minimum BSA concentration (i.e., 0.25%) significantly improved sperm motility (89.05% and 86.63%, respectively) compared to the controls (20.39 and 28.48%, respectively). Similar increases were also noted in progressively motile sperm (PRG, %), the curvilinear velocity of sperm (VCL, µm s), and the amplitude of lateral head displacement (ALH, µm). A similar trend in CASA parameters was also noted when casein was added to Woynarovich and Perchec solutions at a concentration of 0.25%. We concluded that 0.25% doses of each of the proteins were sufficient to prevent sperm adhesion to glass slides, and they can be used in research on ide sperm motility measurements.
本研究的目的是比较四种激活溶液（AS）——沃伊纳罗维奇溶液、拉恩施泰纳溶液、库哈尔奇克溶液和佩尔切克溶液——添加0.5%牛血清白蛋白（BSA）用于圆腹雅罗鱼精子激活并使用计算机辅助精子分析（CASA）系统进行分析。研究发现，在pH值7.4 - 9.0和渗透压160 - 200 mOsm/kg范围内，每种激活溶液均可激活圆腹雅罗鱼精子。实验期间还分析了添加浓度为0.25%、0.5%、1.0%和2.0%的BSA和酪蛋白的沃伊纳罗维奇溶液和佩尔切克溶液的效果。这两种未添加蛋白质的激活溶液（纯溶液）作为对照。与对照（分别为20.39%和28.48%）相比，添加最低BSA浓度（即0.25%）的沃伊纳罗维奇溶液和佩尔切克溶液显著提高了精子活力（分别为89.05%和86.63%）。在进行性运动精子（PRG，%）、精子曲线速度（VCL，µm/s）和头部侧向位移幅度（ALH，µm）方面也观察到类似的增加。当向沃伊纳罗维奇溶液和佩尔切克溶液中添加浓度为0.25%的酪蛋白时，在CASA参数方面也观察到类似趋势。我们得出结论，每种蛋白质0.25%的剂量足以防止精子粘附在载玻片上，可用于圆腹雅罗鱼精子活力测量的研究。
