Saraswat S, Rout P K, Kharche S D, Jindal S K, Goel A K
ICAR-Central Institute for Research on goats, Mathura, India.
Reprod Domest Anim. 2016 Dec;51(6):1049-1054. doi: 10.1111/rda.12774. Epub 2016 Sep 21.
During the last decades, physiological effects of oestrogens have been increasingly explored by scientists and biotechnologists. Estrogens exert a wide range of effects on a large variety of cell types. Oestrogen and its receptors are essential for sexual development and reproduction. Estrogen receptor alpha is a nuclear receptor activated by the hormone oestrogen. In male, ERα is encoded by the gene estrogen receptor gene 1 (ESR1), responsible for better fertility. The ESR1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of ESR1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. Therefore, this is a good startby to study the expression pattern of estrogen receptor 1 gene in high-fertile (G1) and low-fertile (G2) bucks of Jamunapari and Barbari breeds identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the tissues by TRIzol method. The identification and expression pattern of caprine ESR1 gene was analysed by real-time PCR (Roche LC-480). Our work shows that the relative quantification by RT-PCR indicates more fold in head of epididymis as compared to spleen of caprine ESR1 gene. Furthermore, the RT-PCR indicated that fertile bucks of Jamunapari breed have more fold value as compared to Barbari breed in respect of reproductive organ.
在过去几十年中，科学家和生物技术专家对雌激素的生理效应进行了越来越多的探索。雌激素对多种细胞类型具有广泛的影响。雌激素及其受体对于性发育和生殖至关重要。雌激素受体α是一种由雌激素激活的核受体。在雄性中，ERα由雌激素受体基因1（ESR1）编码，对提高生育能力至关重要。ESR1参与精子从睾丸运输到附睾头部过程中管腔液的重吸收，这对精子在附睾储存期间的存活和成熟很重要。ESR1的缺失会导致附睾精子含量减少、精子活力和受精能力降低。因此，这是一个很好的开端，通过研究基于精液质量性状和生育力试验鉴定出的Jamunapari和Barbari品种高生育力（G1）和低生育力（G2）公羊中雌激素受体1基因的表达模式。通过TRIzol法从组织中提取RNA。通过实时PCR（罗氏LC-480）分析山羊ESR1基因的鉴定和表达模式。我们的工作表明，RT-PCR的相对定量显示，山羊ESR1基因在附睾头部的倍数比在脾脏中更多。此外，RT-PCR表明，就生殖器官而言，Jamunapari品种的可育公羊的倍数比Barbari品种更高。
