基质细胞衍生因子-1的表达与骨肉瘤的不良预后相关。

SDF-1 Expression is Associated with Poor Prognosis in Osteosarcoma.

作者信息

Yu Dangen, Lv Fei, Zhang Jianhe, Li Hongjie

机构信息

Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei, P. R. China.

Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei, P. R. China

出版信息

Ann Clin Lab Sci. 2016 Sep;46(5):508-14.

PMID:27650618

Abstract

BACKGROUND

Stromal cell-derived factor-1 (SDF-1) expression has been reported to be a predictor of poor clinical symptoms in certain types of cancer. Vascular endothelial growth factor (VEGF) is a well-known factor that mediates the micro-angiogenesis of solid tumors, and SDF-1 mediated expression of VEGF may promote tumor growth and metastasis, resulting in poor clinical outcome. Therefore, we explored the expression levels of SDF-1 and VEGF in patients with osteosarcoma in order to determine the association between their expression levels and unfavorable outcomes.

METHODS

A total of 54 patients with osteosarcoma were included in the current study. The protein expression levels of SDF-1 and VEGF were evaluated on immunohistochemical and immunofluorescence staining. The correlation between the expression levels of SDF-1 and VEGF and their association with clinical parameters were analyzed using the Pearson chi-square test and the Spearman-rho test. Univariate and multivariate Cox regression analyses were used to identify potential prognostic factors. The Kaplan-Meier method was employed to analyze overall survival.

RESULT

Low SDF-1 and VEGF expression levels were detected in 20.4% (11 of 54) and 22.2% (12 of 54) of the patients with osteosarcoma, respectively; moderate expression was detected in 35.2% (19 of 54) and 37.0% (20 of 54) of the patients, respectively; and high expression was detected in 44.4% (24 of 54) and 40.7% (22 of 54) of the patients, respectively. Protein levels of both SDF-1 and VEGF were significantly associated with the histologic grade (p=0.004 and p=0.042 respectively), the presence of metastasis (p=0.009 and p=0.028 respectively), and Enneking staging (p<0.001 and p=0.003 respectively). The association between expression levels of SDF-1and VEGF had a significantly positive correlation (p<0.001and r=0.618). The expression levels of both SDF-1 and VEGF were significantly associated with shorter overall survival on univariate analysis; however, the association was significant for SDF-1 expression alone in the multivariate analysis (p=0.26, hazard ratio =2.640 [1.124-6.200]).

CONCLUSION

SDF-1 and VEGF expression levels were both significantly associated with osteosarcoma, and SDF-1 expression is a potential independent prognostic indicator in patients with osteosarcoma.

摘要

背景

据报道，基质细胞衍生因子-1（SDF-1）的表达是某些类型癌症临床症状不佳的预测指标。血管内皮生长因子（VEGF）是一种众所周知的介导实体瘤微血管生成的因子，SDF-1介导的VEGF表达可能促进肿瘤生长和转移，导致临床预后不良。因此，我们探讨了骨肉瘤患者中SDF-1和VEGF的表达水平，以确定它们的表达水平与不良预后之间的关联。

方法

本研究共纳入54例骨肉瘤患者。通过免疫组织化学和免疫荧光染色评估SDF-1和VEGF的蛋白表达水平。使用Pearson卡方检验和Spearman秩相关检验分析SDF-1和VEGF表达水平之间的相关性及其与临床参数的关联。采用单因素和多因素Cox回归分析来确定潜在的预后因素。采用Kaplan-Meier法分析总生存期。

结果

骨肉瘤患者中，分别有20.4%（54例中的11例）和22.2%（54例中的12例）检测到低水平的SDF-1和VEGF表达；分别有35.2%（54例中的19例）和37.0%（54例中的20例）检测到中等水平的表达；分别有44.4%（54例中的24例）和40.7%（54例中的22例）检测到高水平的表达。SDF-1和VEGF的蛋白水平均与组织学分级（分别为p=0.004和p=0.042）、转移情况（分别为p=0.009和p=0.028）以及Enneking分期（分别为p<0.001和p=0.003）显著相关。SDF-1和VEGF表达水平之间存在显著正相关（p<0.001，r=0.618）。单因素分析显示，SDF-1和VEGF的表达水平均与较短的总生存期显著相关；然而，多因素分析中仅SDF-1表达的相关性显著（p=0.26，风险比=2.640[1.124 - 6.200]）。