Zhou Xuelin, Choi Pou Seng, Yang Jia-Ming, Or Penelope M Y, Hoi Pui Man, Lee Simon M Y, Leung George P H, Ngai Sai Ming, Kong Siu Kai, Ho Ho Pui, Wong Melody Y M, Chan Shun Wan, Yeung John H K, Kwan Yiu Wa
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
Institute of Chinese Medical Sciences, University of Macau, Macao, China.
Springerplus. 2016 Aug 30;5(1):1438. doi: 10.1186/s40064-016-3115-3. eCollection 2016.
This study reported a comprehensive approach (comparing the extraction yields, chemical profiles, antioxidant properties and CYP450-inhibitory effects) to evaluated the effectiveness of various extraction methods [microwave-assisted extraction using water (MAE-W), heat reflux extraction using water (HRE-W), ultrasonic extraction using 70 % ethanol and ultrasonic extraction using ethanol (UE-E)] for Huang-Qin (HQ), the dried root of Scutellaria baicalensis Georgi.
The HQ extraction efficiency by MAE-W was the best. The chemical profiles of extracts obtained using HRE-W and MAE-W were similar; whereas more flavones but less flavone glycosides were detected in the UE-E extract. There was no difference in the antioxidant properties among different extracts. In vitro human liver microsome assays illustrated that all extracts possessed herb-drug interaction potentials but the UE-E extract are shown with a potent interaction with CYP3A4-metabolized drugs.
MAE-W is a favorable method for the preparation of HQ extracts based on extraction yield, pharmacological properties and safety.
本研究报告了一种综合方法（比较提取率、化学轮廓、抗氧化特性和细胞色素P450抑制作用），以评估多种提取方法[水相微波辅助提取（MAE-W）、水相热回流提取（HRE-W）、70%乙醇超声提取和乙醇超声提取（UE-E）]用于提取黄芩（HQ）（黄芩干燥根）的有效性。
MAE-W的HQ提取效率最佳。HRE-W和MAE-W获得的提取物的化学轮廓相似；而UE-E提取物中检测到更多黄酮但黄酮苷较少。不同提取物的抗氧化特性没有差异。体外人肝微粒体试验表明，所有提取物都具有药-药相互作用潜力，但UE-E提取物与CYP3A4代谢药物表现出强烈相互作用。
基于提取率、药理特性和安全性，MAE-W是制备HQ提取物的一种良好方法。
