Park Hyeon Soo, Park Kwang Il, Hong Gyeong Eun, Nagappan Arulkumar, Lee Ho Jeong, Kim Eun Hee, Lee Won Sup, Shin Sung Chul, Seo On Nuri, Won Chung Kil, Cho Jae Hyeon, Kim GonSup
Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Gyeongsang National University, Gazwa, Jinju 660-701, Republic of Korea.
Department of Biological Science, Center for Colon Cancer Research, University of South Carolina, Columbia, SC 29208, USA.
J Ethnopharmacol. 2014 Aug 8;155(1):847-51. doi: 10.1016/j.jep.2014.05.053. Epub 2014 Jun 6.
Scutellaria baicalensis Georgi, commonly known as skullcaps, and it has been widely used as traditional therapeutic herb in several eastern Asia including Korea, China and Japan because of its remarkable anti-inflammatory and anti-cancer effects. Our study focuses on the anti-metastatic effects of Scutellaria baicalensis Georgi in hepatocellular carcinoma (HCC).
Methanol extract of Scutellaria baicalensis Georgi was examined for identification of its composition by HPLC-MS/MS. The extract was evaluated for the anti-metastasis activity using HepG2 hepatocellular carcinoma cells via immunoblotting and RT-PCR. For mechanical study, specific Forkhead Box M1 (FOXM1) vector was transfected to HepG2 cells.
Scutellaria baicalensis Georgi potentially inhibited proliferation of HepG2 cells dose dependently. Scutellaria baicalensis Georgi decreased metastasis through the regulation of matrix metalloproteinase 2 (MMP-2) and FOXM1 activities at the transcription and translation levels.
The results of the present study suggest that Scutellaria baicalensis Georgi could be a potent chemotherapeutic agent against HCC. Its clinical use guarantee for further study and individual flavonoids from Scutellaria baicalensis Georgi should also be investigated.
黄芩，俗称灯盏花，因其显著的抗炎和抗癌作用，在包括韩国、中国和日本在内的几个东亚国家被广泛用作传统治疗草药。我们的研究聚焦于黄芩对肝细胞癌（HCC）的抗转移作用。
采用高效液相色谱-串联质谱法（HPLC-MS/MS）对黄芩甲醇提取物的成分进行鉴定。通过免疫印迹和逆转录-聚合酶链反应（RT-PCR），利用HepG2肝癌细胞评估该提取物的抗转移活性。为进行机制研究，将特异性叉头框M1（FOXM1）载体转染至HepG2细胞。
黄芩可剂量依赖性地潜在抑制HepG2细胞增殖。黄芩通过在转录和翻译水平调节基质金属蛋白酶2（MMP-2）和FOXM1的活性来降低转移。
本研究结果表明，黄芩可能是一种有效的抗肝癌化疗药物。其临床应用保证有待进一步研究，同时也应研究黄芩中的个别黄酮类化合物。
